ALLOCATION OF EPIBLAST CELLS TO GERM LAYER DERIVATIVES DURING MOUSE GASTRULATION AS STUDIED WITH A RETROVIRAL VECTOR
ALLOCATION OF EPIBLAST CELLS TO GERM LAYER DERIVATIVES DURING MOUSE GASTRULATION AS STUDIED WITH A RETROVIRAL VECTOR
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DOI：
10.1002/dvg.1020170105
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发表时间：
1995-01-01
期刊：
影响因子：
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通讯作者：
PEDERSEN, RA
中科院分区：
文献类型：
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作者：
CAREY, FJ;LINNEY, EA;PEDERSEN, RA
胚胎外胚层或外胚层是小鼠胚胎原肠胚形成期间形成的三个初级胚层的来源。以前的研究已经调查了上胚层细胞的命运，在早期原肠胚形成阶段使用细胞谱系的克隆分析和在晚期原肠胚形成阶段使用移植标记的移植物。在这项研究中，我们研究了晚期原肠胚形成阶段上胚层的命运，使用基于逆转录病毒载体编码的大肠杆菌lacZ基因的克隆分析。我们发现，通过减少注射到每个胚胎中的病毒悬浮液的体积，有可能实现单一感染事件。我们分析了20个在条纹晚期和21个在头褶期单独感染的胚胎，发现每个胚胎中只有一个胚层中的克隆后代。这些结果表明，在小鼠胚胎中，上胚层祖细胞分配到一个胚层的命运发生在原肠胚形成晚期。(C)1995 Wiley-Liss，Inc.
The embryonic ectoderm, or epiblast, is the source of the three primary germ layers that form during gastrulation in the mouse embryo. Previous studies have investigated the fate of epiblast cells in early gastrulation stages using clonal analysis of cell lineage and in late gastrulation stages using transplantation of labeled grafts. In this study, we studied the fate of late gastrulation stage epiblast using a clonal analysis based on a retroviral vector encoding the Escherichia coli lacZ gene. We found that by reducing the volume of viral suspension injected into each embryo, it was possible to achieve single infectious events. Our analysis of 20 embryos singly infected at the late streak stage and 21 at the head fold stage revealed clonal descendants in only a single germ layer in each embryo, These results indicate that allocation of epiblast progenitors to a single germ layer fate has occurred by late gastrulation in mouse embryos. (C) 1995 Wiley-Liss, Inc.