Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro
Chemical and biological comparison of different parts of Paeonia suffruticosa (Mudan) based on LCMS-IT-TOF and multi-evaluation in vitro
复制标题
基于LCMS-IT-TOF及体外多重评价的牡丹不同部位化学生物学比较
DOI：
10.1016/j.indcrop.2019.112028
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发表时间：
2020-02-01
影响因子：
5.9
通讯作者：
Geng, Chang-An
中科院分区：
文献类型：
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作者：
Pan, Yang;Gao, Zhen;Geng, Chang-An
白芍牡丹是一种著名的药用和观赏植物，在中国广泛种植。在传统中医体系中，只有牡丹皮（牡丹皮）被用于生产生药。本研究旨在通过LCMS-IT-TOF分析和体外生物测定，系统比较苦参不同部位（根皮、根芯、老茎、幼茎和叶）的化学特征和生物活性。通过紫外吸收光谱、MS/MS碎片化以及与参比化合物的比较，共鉴定了55个成分，包括15种单萜苷、13种没食子酸葡萄糖、7种没食子酸衍生物、4种苯乙酮、6种黄酮类化合物和10种其他化合物。有趣的是，一个新的单萜，氧代芍药苷元，被分配到其MSn和UV模式。定量分析表明，苦参的主要成分为没食子酸(2)(5439.1 +/- 90.2 μ g/g)，芍药苷（13）（16378.9 +/- 195.4 μ g/g）、根核（17130.2 +/- 125.9 μ g/g）和叶（16682.9 +/- 181.2 μ g/g），苯甲酰芍药苷（46）和芍药酚（49）（2360.1 +/- 41.4和10142.3 +/- 252.5 μ g/g）和根核（2249.5 +/- 21.7和10441.6 +/- 363.9 μ g/g）。根皮和根芯化学成分相似，茎叶化学成分差异明显。测定五部分对四种神经递质受体（MT1、MT2、5-HT1A和5-HT2C）的激动作用，以及对α -葡萄糖苷酶、PTP1B和DPPIV的降糖作用。除根皮外，根核、茎和叶也显示出令人着迷的活性。根皮提取率（35.4% +/- 2.18）是根芯提取率（18.4% +/- 0.81）的2倍，支持传统应用。本研究为进一步认识沙参不同部位间的化学差异及综合利用提供了新的视角。
Paeonia suffruticosa Andr. (Mudan) as a famous medicinal and ornamental plant is widely cultivated in China. In the traditional Chinese medicine (TCM) system, only the root bark (Moutan Cortex) is used in the production of crude drugs. The present study aimed to systematically compare the chemical profiles and biological activities of different parts (root bark, root core, old stems, young stems, and leaves) of P. suffruticosa by LCMS-IT-TOF analysis and bioassay in vitro. In total, 55 constituents involving 15 monoterpene glycosides, 13 galloyl glucoses, seven gallic acid derivatives, four acetophenones, six flavonoids, and ten other compounds were characterized according to their UV absorptions, MS/MS fragmentation, and comparing with reference compounds. Interestingly, one new monoterpene, oxypaeoniflorgenin, was assigned by its MSn and UV patterns. Quantitative analysis demonstrated that gallic acid (2) mainly in leaves (5439.1 +/- 90.2 mu g/g), paeoniflorin (13) mainly in root bark (16,378.9 +/- 195.4 mu g/g), root core (17,130.2 +/- 125.9 mu g/g), and leaves (16,682.9 +/- 181.2 mu g/g), and benzoylpaeoniflorin (46) and paeonol (49) mainly in root bark (2360.1 +/- 41.4 and 10,142.3 +/- 252.5 mu g/g) and root core (2249.5 +/- 21.7 and 10,441.6 +/- 363.9 mu g/g) were the main constituents in P. suffruticosa. The chemical constituents of root bark and root core were similar and obviously different from the stems and leaves. All the five parts were assayed for their agonistic effect on four neurotransmitter receptors (MT1, MT2, 5-HT1A, and 5-HT2C) and antidiabetic potency on alpha-glucosidase, PTP1B, and DPPIV. Besides root bark, the root core, stems, and leaves were also revealed with fascinating activity in this study. The extraction rate of root bark (35.4% +/- 2.18) was two times higher than that of root core (18.4% +/- 0.81), supporting the traditional application. This investigation provides new perspectives for understanding the chemical divergence between different parts of P. suffruticosa and their comprehensive utilization.