Chloramine-t for “no-carrier-added” labelling of aromatic biomolecules with bromine-75,77
Chloramine-t for “no-carrier-added” labelling of aromatic biomolecules with bromine-75,77
复制标题
氯胺-t 用于用溴-75,77 对芳香族生物分子进行“无载体添加”标记
DOI：
10.1002/jlcr.2580180908
复制
发表时间：
1981
影响因子：
1.8
通讯作者：
H. Coenen
中科院分区：
文献类型：
--
作者：
G. Petzold;H. Coenen
研究了氯胺- t （CAT）对短寿命同位素溴-77 （T1/2 = 56 h）和溴-75 （T1/2 = 98 min）的芳香族化合物“无载体添加”标记的适用性。在水溶液中，以酪氨酸为模型底物，确定了pH、CAT浓度、反应时间和溴载体的最佳反应条件。在pH为1，CAT浓度为10−3 M的条件下，在几秒钟内获得了最高的放射化学产率（>85%），在pH为7的条件下，在10−2 M条件下，2.5分钟后获得了65%的最大放射化学产率。对一些模型肽的研究表明，只有酪氨酸残基被溴化，其产率与游离酪氨酸相似。用CAT法对α-甲基酪氨酸、苯丙氨酸、尿嘧啶、胞嘧啶、苯酚等芳香族分子进行了放射性溴化处理，收率达90%。用无线电高效液相色谱法对标记产物进行分离和鉴定。
The applicability of chloramine-T (CAT) was examined for “no-carrier-added” labelling of aromatic compounds with the short-lived isotopes bromine-77 (T1/2 = 56 h) and bromine-75 (T1/2 = 98 min). In aqueous solution the optimum reaction conditions with respect to pH, concentration of CAT, reaction time and added brominecarrier were determined using tyrosine as a model substrate. Highest radiochemical yields (>85%) were obtained at pH 1 within a few seconds at a CAT concentration of 10−3 M. At pH 7 a maximum radiochemical yield of 65% was achieved with 10−2 M after 2.5 minutes. Studies with some model peptides showed that only the tyrosyl residues were brominated and similar yields were obtained as in free tyrosine. Some aromatic molecules such as α-methyl-tyrosine, phenylalanine, uracil, cytosine, and phenol were radiobrominated by the CAT method with yields up to 90%. Separation and identification of the labelled products were achieved by means of radio high performance liquid chromatography.