A role of CB1R in inducing θ-rhythm coordination between the gustatory and gastrointestinal insula.
A role of CB1R in inducing θ-rhythm coordination between the gustatory and gastrointestinal insula.
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DOI：
10.1038/srep32529
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发表时间：
2016-09-01
影响因子：
4.6
通讯作者：
Toyoda H
中科院分区：
文献类型：
--
作者：
Kang Y;Sato H;Saito M;Yin DX;Park SK;Oh SB;Bae YC;Toyoda H
花生四烯酸酰胺（AEA）和N-油酰乙醇胺（OEA）分别在禁食和饱腹时在肠道和大脑中产生。随后，AEA通过激活大麻素1型受体（CB 1 R）促进食物摄入，而OEA通过激活过氧化物酶体增殖物激活受体-α（PPARα）和/或G蛋白偶联受体119（GPR 119）减少食物摄入。在禁食期间，与味觉神经元（Gu-I）嘴侧相邻的自主神经元（GI-Au-I）的胃肠道区域中的神经元活动增加，从而增强食欲，而Gu-I中的鲜味和甜味感觉增强了GI-Au-I中的食欲，强烈表明Gu-I和GI-Au-I之间存在神经相互作用，其根据AEA和OEA的浓度而变化。然而，这种可能性从未被调查过。在大鼠脑片制备中，我们用电压敏感染料成像证明，通过AEA激活CB 1 Rs诱导Gu-I中的θ节律振荡同步，其传播到GI-Au-I，但在其尾端停止，显示振荡协调。CB 1 R拮抗剂或OEA通过激活GPR 119消除了AEA诱导的振荡。我们的研究结果表明，Gu-I和GI-Au-I之间的神经协调是由CB 1 R和GPR 119之间的相反活动产生或抑制的。这种机制可能与基于味觉识别的摄食行为有关。
Anandamide (AEA) and N-oleoylethanolamine (OEA) are produced in the intestine and brain during fasting and satiety, respectively. Subsequently, AEA facilitates food intake via activation of cannabinoid type-1 receptors (CB1Rs) while OEA decreases food intake via activation of peroxisome proliferator-activated receptor-α (PPARα) and/or G-protein-coupled receptor 119 (GPR119). Neuronal activity in the gastrointestinal region of the autonomic insula (GI-Au-I) that rostrally adjoins the gustatory insula (Gu-I) increases during fasting, enhancing appetite while umami and sweet taste sensations in Gu-I enhances appetite in GI-Au-I, strongly suggesting the presence of a neural interaction between the Gu-I and GI-Au-I which changes depending on the concentrations of AEA and OEA. However, this possibility has never been investigated. In rat slice preparations, we demonstrate with voltage-sensitive dye imaging that activation of CB1Rs by AEA induces θ-rhythm oscillatory synchronization in the Gu-I which propagates into the GI-Au-I but stops at its caudal end, displaying an oscillatory coordination. The AEA-induced oscillation was abolished by a CB1R antagonist or OEA through activation of GPR119. Our results demonstrate that the neural coordination between the Gu-I and GI-Au-I is generated or suppressed by the opposing activities between CB1R and GPR119. This mechanism may be involved in the feeding behavior based on taste recognition.
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