THE PROPERTIES OF T-ANTIGENS EXTRACTED FROM GROUP A HEMOLYTIC STREPTOCOCCI
THE PROPERTIES OF T-ANTIGENS EXTRACTED FROM GROUP A HEMOLYTIC STREPTOCOCCI
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DOI：
10.1084/jem.84.5.449
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发表时间：
1946-01-01
影响因子：
15.3
通讯作者：
DOLE, VP
中科院分区：
文献类型：
--
作者：
LANCEFIELD, RC;DOLE, VP
A 组溶血性链球菌的 T 抗原已通过用胃蛋白酶或胰蛋白酶消化细菌细胞而获得可溶形式。从 1 型菌株中可以很容易地提取大量的这种抗原，但只能提取少量的抗原。可以从其他类型的菌株中获得。 T 抗原，准备好的。以这种方式从 1 型菌株中通过化学沉淀和进一步的酶消化进行部分纯化。在 pH 7 下通过电泳分离活性组分（显然本质上是蛋白质）。分离的材料在相同 pH 下汇集和分析，仅给出一个峰。紫外线。吸收显示典型的蛋白质曲线，最大值在378mμ处，最小值在254mμ处。该物质的等电点约为pH 4.5。微量化学分析得出N = 14.25%，C = 50.11%，H = 7.42%，灰分= 1.9%。尽管T抗原能抵抗蛋白水解酶和核糖核酸酶的消化，但它很容易被热灭活，特别是在酸性介质和强盐溶液中。这种纯化的 T 物质的血清学活性在暴露于紫外线后消失。辐射。在纯化的 T 物质中添加酵母核酸或在纯化程度较低的 T 制剂中存在核糖核酸。防止暴露于紫外线期间血清学活性的丧失。辐射。通过超速离心机的分析表明该材料是多分散的，因此可能不纯。 T物质的可溶形式在沉淀素反应、补体固定、T凝集抑制以及感染时作为抗原具有活性。变成兔子。产生的抗体不能保护小鼠免受含有相同 T 抗原的溶血性链球菌强毒株的感染。可溶形式的T抗原的免疫学特异性与其来源的完整链球菌中的T抗原相同。
T antigens of group A hemolytic streptococci have been obtained in soluble form by digestion of the bacterial cells with pepsin or trypsin. Large quantities of this antigen were readily extracted from type 1 strains, whereas only small amts. could be obtained from strains of other types. The T antigen, prepd. in this way from a type 1 strain, was partially purified by chemical precipitation and further enzymatic digestion. An active fraction, apparently protein in nature, was separated electro-phoretically at pH 7. The separated material, pooled and analyzed at the same pH, gave only a single peak. U.-v. absorption showed a typical protein curve with a maximum at 378 m[mu] and a minimum at 254 m[mu]. The isoelectric point of this substance was about pH 4.5. Microchemical analysis gave N = 14.25%, C = 50.11%, H = 7.42%, and ash = 1.9%. Although the T antigen was resistant to digestion with proteolytic enzymes and ribonuclease, it was readily inactivated by heat, especially in acid media and in strong salt solns. The serological activity of this purified T substance was lost after exposure to u.-v. radiation. The addition of yeast nucleic acid to the purified T substance or the presence of ribonucleic acid in less purified T prepns. prevented the loss of serological activity during exposure to u.-v. radiation. Analysis by means of the ultracentrifuge showed that the material was polydisperse and therefore probably impure. The soluble form of the T substance was active in the precipitin reaction, in the fixation of complement, in inhibition of T agglutination, and as an antigen when injd. into rabbits. The antibodies produced did not protect mice against infection with virulent strains of hemolytic streptococci containing the same T antigen. The immunological specificity of T antigen in soluble form is the same as that of the T antigen in the intact streptococcus from which it was derived.