Alterations in red blood cell volume and hemoglobin concentration, viscoelastic properties, and mechanical fragility caused by continuous flow pumping in calves
Alterations in red blood cell volume and hemoglobin concentration, viscoelastic properties, and mechanical fragility caused by continuous flow pumping in calves
复制标题
犊牛连续流动泵送引起的红细胞体积和血红蛋白浓度、粘弹性和机械脆性的变化
DOI：
10.1111/j.1525-1594.2011.01317.x
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发表时间：
2011
影响因子：
2.4
通讯作者：
S.Takatani
中科院分区：
文献类型：
--
作者：
N.Yokoyama;D.Sakota;E.Nagaoka;S.Takatani
在这项研究中，我们分析了平均红细胞体积（MCV），平均红细胞血红蛋白浓度（MCHC），红细胞（RBC）的动态变形性和机械脆性的变化在5个雄性荷斯坦犊牛（体重：95.6 ± 10.8 kg）的循环部分支持与一种新的磁悬浮体外离心血泵MedTech Dispo。 泵血后1h，MCV较泵血前增加1.064 ± 0.006倍，MCHC较泵血前减少0.906±0.050倍（P< 0.05）。 通过循环反向剪切流剪切的变形指数L/W（其中L和W分别是二维RBC图像的长轴和短轴）增加，表明栗送1小时的RBC表现出更大的弹性特征（P<0.05）。 此外，当用25.38 Pa的均匀剪切应力剪切泵送的血细胞30 min时，与对照血液相比，溶血水平显著降低，因为更脆弱的RBC被泵送破坏，留下较不脆弱的RBC。 暴露于连续流动的RBC的所有这些特征类似于具有较大MCV、较低MCHC、较高弹性和较低脆性的年轻RBC的那些特征。总之，在连续流动泵送期间，对于由连续流动血泵（CFBP）产生的机械剪切应力具有相对较低的溶血阈值的RBC首先被破坏，并且在CFBP应用的早期阶段从循环中去除，从而留下较不脆弱和较强的RBC。
In this study, we have analyzed the changes in mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), and the dynamic deformability and mechanical fragility of red blood cells (RBCs) in five male Holstein calves (body weight: 95.6 ± 10.8 kg) whose circulation was partially supported with a novel magnetically levitated extracorporeal centrifugal blood pump MedTech Dispo. One hour after the pumping has started, the MCV increased and the MCHC decreased by 1.064 ± 0.006 and 0.906 ± 0.050 times, respectively, as compared with those of the prepumped blood (P< 0.05). The deformability index L/W, where L and W are the long and short axes of the two‐dimensional RBC images, respectively, sheared by a cyclically reversing shear flow increased indicating that the RBCs pumped for 1 h exhibited more elastic characteristics (P< 0.05). In addition, when the pumped blood cells were sheared for 30 min with a uniform shear stress of 25.38 Pa, the hemolysis level decreased dramatically as compared with the control blood, as more fragile RBCs were destroyed by pumping, leaving behind less fragile RBCs. All these characteristics of the RBCs exposed to continuous flow resemble those of young RBCs having larger MCV, lower MCHC, higher elasticity, and lower fragility. In conclusion, during continuous flow pumping, the RBCs having relatively lower threshold for hemolysis to mechanical shear stress generated by continuous flow blood pump (CFBP) are destroyed first and removed from circulation in the early stage of application of CFBP, thus leaving behind less fragile and stronger RBCs.