The biological effects of subacute inhalation of diesel exhaust following addition of cerium oxide nanoparticles in atherosclerosis-prone mice.
The biological effects of subacute inhalation of diesel exhaust following addition of cerium oxide nanoparticles in atherosclerosis-prone mice.
复制标题
DOI：
10.1016/j.envres.2012.03.004
复制
发表时间：
2012-05
影响因子：
8.3
通讯作者：
Miller MR
中科院分区：
文献类型：
--
作者：
Cassee FR;Campbell A;Boere AJ;McLean SG;Duffin R;Krystek P;Gosens I;Miller MR
氧化铈（CeO 2）纳米颗粒提高了燃料的燃烧效率，然而，人们对车辆排放物的改变对健康的影响知之甚少。动脉粥样硬化易感性载脂蛋白E敲除（ApoE−/−）小鼠通过吸入暴露于稀释的废气（1.7 mg/m3，20，60或180 min，5天/周，持续4周），该废气来自使用标准柴油燃料（DE）或含有9 ppm氧化铈纳米颗粒（DCeE）的相同柴油燃料的发动机。评估血液学指标、临床化学、动脉粥样硬化负荷、炎性细胞因子组织水平和主要器官病理学的变化。在燃料中添加CeO 2导致排气中颗粒的数量（30%）和表面积（10%）减少，而气态共污染物增加（6-8%）。然而，DE暴露后动脉粥样硬化斑块的大小和复杂性有增加的趋势，这在DCeE组中并不明显。两种给药均未诱导明显的血液学或病理学改变体征。然而，促炎性细胞因子的水平调制在脑区和肝脏以下DCeE曝光。这些结果意味着，添加CeO 2纳米颗粒的燃料减少排气中的颗粒数量，并可能减少与暴露于标准柴油燃料相关的动脉粥样硬化负担。根据对生物学参数进行的广泛评估，添加铈的唯一相关影响是中枢神经系统区域中细胞因子水平略微升高。总的来说，使用铈作为燃料添加剂可能是限制汽车尾气对健康影响的一种潜在有用的方法。然而，需要进一步的测试，以确保这种方法不会与慢性炎症反应相关，最终可能导致长期的健康影响。添加到柴油中的CeO 2纳米颗粒减少了废气中的颗粒数量。ApoE小鼠的动脉粥样硬化与暴露于标准柴油燃料有关。柴油中的CeO 2纳米颗粒在小鼠脑中诱导细胞因子。
Cerium oxide (CeO2) nanoparticles improve the burning efficiency of fuel, however, little is known about health impacts of altered emissions from the vehicles. Atherosclerosis-prone apolipoprotein E knockout (ApoE−/−) mice were exposed by inhalation to diluted exhaust (1.7 mg/m3, 20, 60 or 180 min, 5 day/week, for 4 weeks), from an engine using standard diesel fuel (DE) or the same diesel fuel containing 9 ppm cerium oxide nanoparticles (DCeE). Changes in hematological indices, clinical chemistry, atherosclerotic burden, tissue levels of inflammatory cytokines and pathology of the major organs were assessed. Addition of CeO2 to fuel resulted in a reduction of the number (30%) and surface area (10%) of the particles in the exhaust, whereas the gaseous co-pollutants were increased (6–8%). There was, however, a trend towards an increased size and complexity of the atherosclerotic plaques following DE exposure, which was not evident in the DCeE group. There were no clear signs of altered hematological or pathological changes induced by either treatment. However, levels of proinflammatory cytokines were modulated in a brain region and liver following DCeE exposure. These results imply that addition of CeO2 nanoparticles to fuel decreases the number of particles in exhaust and may reduce atherosclerotic burden associated with exposure to standard diesel fuel. From the extensive assessment of biological parameters performed, the only concerning effect of cerium addition was a slightly raised level of cytokines in a region of the central nervous system. Overall, the use of cerium as a fuel additive may be a potentially useful way to limit the health effects of vehicle exhaust. However, further testing is required to ensure that such an approach is not associated with a chronic inflammatory response which may eventually cause long-term health effects. ► CeO2 nanoparticles added to diesel decreases the number of particles in exhaust. ► Atherosclerosis is associated with exposure to standard diesel fuel in ApoE mice. ► CeO2 nanoparticles in diesel induce cytokines in the brain of mice.
登录
查看更多内容
影响因子：
5
作者：
Ma, Jane Y.;Zhao, Hongwen;Ma, Joseph K.
通讯作者：
Ma, Joseph K.
影响因子：
10
作者：
Araujo JA;Nel AE
通讯作者：
Nel AE
影响因子：
3.8
作者：
Campen, Matthew J.;Lund, Amie K.;McDonald, Jacob D.
通讯作者：
McDonald, Jacob D.
影响因子：
1.8
作者：
HADOKE, P;WAINWRIGHT, CL;GIDDINGS, MJ
通讯作者：
GIDDINGS, MJ
影响因子：
5.3
作者：
Bai N;Kido T;Suzuki H;Yang G;Kavanagh TJ;Kaufman JD;Rosenfeld ME;van Breemen C;Eeden SF
通讯作者：
Eeden SF