THE DROSOPHILA SLOPPY PAIRED LOCUS ENCODES 2 PROTEINS INVOLVED IN SEGMENTATION THAT SHOW HOMOLOGY TO MAMMALIAN TRANSCRIPTION FACTORS
THE DROSOPHILA SLOPPY PAIRED LOCUS ENCODES 2 PROTEINS INVOLVED IN SEGMENTATION THAT SHOW HOMOLOGY TO MAMMALIAN TRANSCRIPTION FACTORS
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DOI：
10.1101/gad.6.6.1030
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发表时间：
1992-06-01
影响因子：
10.5
通讯作者：
GEHRING, WJ
中科院分区：
文献类型：
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作者：
GROSSNIKLAUS, U;PEARSON, RK;GEHRING, WJ
草率配对基因座参与果蝇胚胎同色异体计划的建立。我们通过 P 元件介导的增强子检测克隆了草率配对基因座。该基因座由两个基因 slp1 和 slp2 组成，它们在结构和功能上相关。它们属于一类新的推定转录因子，含有在哺乳动物肝细胞转录因子中也发现的叉头结构域。两个转录本的空间表达模式非常相似，表明这两个基因的共同调控。我们通过增强子检测转座子的重新动员恢复了额外的草率配对等位基因。遗传分析表明，这两个基因都有助于分割表型，该表型具有配对规则和片段极性基因的特征，并且它们在功能上相互作用。这两个基因似乎共享一个位于 slp1 上游的增强子元件，该元件作用于近端 slp1 启动子和远端 slp2 启动子。
The sloppy paired locus is involved in the establishment of the metameric body plan of the Drosophila embryo. We have cloned the sloppy paired locus by P-element-mediated enhancer detection. The locus is composed of two genes, slp1 and slp2, that are structurally and functionally related. They belong to a novel class of putative transcription factors containing a fork head domain that has also been found in mammalian hepatocyte transcription factors. The spatial expression patterns of the two transcripts are very similar, suggesting common regulation of the two genes. We recovered additional sloppy paired alleles by remobilization of an enhancer detector transposon. Genetic analysis suggests that both genes contribute to the segmentation phenotype that has characteristics of both, pair-rule and segment polarity genes, and that they interact functionally. The two genes appear to share an enhancer element situated upstream of slp1 that acts on both the proximal slp1 promoter and the distal slp2 promoter.