Single and multistrain rhizobial inocula for pinto and black bean cultivars
Single and multistrain rhizobial inocula for pinto and black bean cultivars
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DOI：
10.1080/01904160500250664
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发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Walley, FL
中科院分区：
文献类型：
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作者：
Kyei-Boahen, S;Nleya, T;Walley, FL
菜豆（Phaseolus vulgaris L.）根瘤菌固氮能力较差，选择亲和寄主品种和根瘤菌菌株组合可提高固氮能力。在生长室实验中，使用五个菜豆品种（三个平托和两个黑豆）评估六种根瘤菌菌株的有效性。随后，我们选择了三个最好的菌株，以评估多菌株接种是否优于单菌株接种在生长室和田间试验。在第一生长室实验中，选择根瘤菌菌株UMR 1899、RCR 3618和USDA 2676，以获得高的接种率、植物干重、地上部氮（N）和N-2固定。在第二个生长室实验中，单个菌株和三种菌株的混合物在评价的参数方面通常没有差异。地上部总氮积累量为172.9 ~ 162.8mg株（-1），其中32.1%~ 33.6%（相当于54.0 ~ 59.2mg株（-1））为固定氮。在田间试验中，植物生物量和种子N-2固定没有不同的接种剂在任何网站。这些结果表明，这三种菌株是同样有效的，多菌株接种剂提供了不一致的优势，单菌株接种剂。
Common bean ( Phaseolus vulgaris L.) is relatively poor in dinitrogen (N-2) fixation, so selecting compatible host cultivar and Rhizobium strain combinations may offer an improvement. The effectiveness of six rhizobial strains was evaluated using five bean cultivars of bean (three pinto and two black bean) in a growth-room experiment. We subsequently selected the three best strains to assess whether multi-strain inoculation had advantages over single-strain inoculation in growth-room and field experiments. In the first-growth-room experiment, Rhizobium strains UMR 1899, RCR 3618, and USDA 2676 were selected for high nodulation, plant dry weight, shoot nitrogen (N), and N-2 fixation. In a second growth-room experiment, the individual strains and a mixture of the three strains generally did not differ in the parameters evaluated. Total shoot N accumulated ranged from 172.9 to 162.8 mg plant(-1) , of which 32.1% to 33.6% (equivalent to 54.0 to 59.2 mg plant(-1)) was fixed. In field experiments, plant biomass and seed N-2 fixed did not differ among the inoculants at any site. These results suggest that the three strains were equally effective and that the multi-strain inoculant offered no consistent advantage over the single-strain inoculants.