Adipose-derived mesenchymal stromal (stem) cells differentiate to osteoblast and chondroblast lineages upon incubation with conditioned media from dental pulp stem cell-derived osteoblasts and auricle cartilage chondrocytes.
Adipose-derived mesenchymal stromal (stem) cells differentiate to osteoblast and chondroblast lineages upon incubation with conditioned media from dental pulp stem cell-derived osteoblasts and auricle cartilage chondrocytes.
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发表时间：
2016
影响因子：
3.2
通讯作者：
A. Carbone;M. Valente;L. Annacontini;S. Castellani;S. Di Gioia;D. Parisi;M. Rucci;G. Belgiovine;C. Colombo;A. Di Benedetto;G. Mori;L. Lo Muzio;A. Maiorella;A. Portincasa;M. Conese
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作者：
A. Carbone;M. Valente;L. Annacontini;S. Castellani;S. Di Gioia;D. Parisi;M. Rucci;G. Belgiovine;C. Colombo;A. Di Benedetto;G. Mori;L. Lo Muzio;A. Maiorella;A. Portincasa;M. Conese
脂肪间充质干细胞分化为成骨细胞或软骨细胞的潜能是有争议的。在这项研究中，我们调查了多能力的潜力，脂肪干细胞分化成脂肪细胞，成骨细胞和软骨细胞谱系时，细胞接种到塑料与培养条件培养基（CM）从分化的细胞types.ADSCs，从吸脂获得，其特征在于间充质和造血标记细胞荧光。通过组织化学方法（分别为油红O染色、番红O和茜素红染色）研究它们向脂肪细胞、成骨细胞和软骨细胞的分化能力。将牙髓干细胞和去分化的耳廓软骨细胞分别向成骨细胞和软骨细胞方向分化，并将从这些培养物中获得的CM用于诱导ADSCs分化。ADSC对间充质标志物（CD 29、CD 105、CD 73、CD 44）呈阳性，但对造血谱系标志物（CD 14、CD 34、CD 45）不呈阳性，并且这种行为从分离到第五代是保守的。虽然ADSC容易在脂肪细胞中分化，但它们不朝向软骨细胞和成骨细胞谱系，这与在诱导后两周分化成三种谱系的骨髓来源的MSC的行为不同。只有用来自培养的软骨细胞和DPSC的CM处理的ADSC产生糖胺聚糖和矿化基质。这些结果表明，ADSC需要从组织环境中补充生长/形态发生因子，以适当分化为中胚层谱系。
The potential of adipose-derived mesenchymal stromal (stem) cells (ADSCs) to differentiate into either osteoblasts or chondrocytes is controversial. In this study we investigated the multicapacity potential of ADSCs to differentiate towards adipocyte, osteoblast, and chondrocyte lineages when cells are seeded onto plastic in comparison with incubation with conditioned media (CM) obtained from differentiated cell types.ADSCs, obtained from liposuctions, were characterized for mesenchymal and hematopoietic markers by cytofluorimetry. Their differentiation capacity towards adipocytes, osteoblasts, and chondrocytes was investigated by histochemistry methods (Oil-Red-O staining, Safranin O and Alizarin Red staining, respectively). Dental pulp stem cells (DPSCs) and dedifferentiated auricle derived-chondrocytes were differentiated towards osteoblastic and chondrocytic lineages respectively, and the CM obtained from these cultures was used to induce differentiation of ADSCs. ADSCs were positive for mesenchymal markers (CD29, CD105, CD73, CD44), but not for hematopoietic lineage markers (CD14, CD34, CD45) and this behavior was conserved from the isolation up to the fifth passage. While ADSCs were readily differentiated in adipocytes, they were not towards chondrocytes and osteoblastic lineages, a behavior different from that of bone marrow-derived MSCs that differentiated into the three lineages at two weeks post-induction. Only ADSCs treated with CM from cultured chondrocytes and DPSCs, produced glycosaminoglycans and mineralized matrix. These results indicate that ADSCs need growth/morphogenic factor supplementation from the tissue environment to be appropriately differentiated to mesodermic lineages.