In vitro demonstration of neural transmission of avian influenza A virus
In vitro demonstration of neural transmission of avian influenza A virus
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DOI：
10.1099/vir.0.80704-0
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发表时间：
2005-04-01
影响因子：
3.8
通讯作者：
Umemura, T
中科院分区：
文献类型：
--
作者：
Matsuda, K;Shibata, T;Umemura, T
流感病毒感染后的神经受累可能很严重。流感病毒从外周神经系统到中枢神经系统的神经运输已通过小鼠模型得到证实。然而，没有直接证据表明神经感染已在体外获得和流感病毒的神经传播机制尚未报道。在这项研究中，利用小鼠背根神经节神经元的区隔化培养物检测了嗜神经型甲型流感病毒的跨神经传播，并将结果与使用假狂犬病毒获得的结果进行了比较，假狂犬病毒是一种已建立神经传播的病毒。这两种病毒都通过轴突到达神经元的细胞体。这是关于甲型流感病毒轴突转运的首次体外报道。此外，通过细胞骨架摄动实验研究了细胞骨架（微管、微丝和中间丝）在流感病毒神经传播中的作用。结果表明，甲型禽流感病毒在神经元中的传播不依赖于微管的完整性，而依赖于中间丝的完整性，而伪狂犬病毒在神经传播中需要两者的完整性。
Neural involvement following infections of influenza viruses can be serious. The neural transport of influenza viruses from the periphery to the central nervous system has been indicated by using mouse models. However, no direct evidence for neuronal infection has been obtained in vitro and the mechanisms of neural transmission of influenza viruses have not been reported. In this study, the transneural transmission of a neurotropic influenza A virus was examined using compartmentalized cultures of neurons from mouse dorsal root ganglia, and the results were compared with those obtained using the pseudorabies virus, a virus with well-established neurotransmission. Both viruses reached the cell bodies of the neurons via the axons. This is the first report on axonal transport of influenza A virus in vitro. In addition, the role of the cytoskeleton (microtubules, microfilaments and intermediate filaments) in the neural transmission of influenza virus was investigated by conducting cytoskeletal perturbation experiments. The results indicated that the transport of avian influenza A virus in the neurons was independent of microtubule integrity but was dependent on the integrity of intermediate filaments, whereas pseudorabies virus needed both for neural spread.