Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis.
Phosphorylation by Cdk1 induces Plk1-mediated vimentin phosphorylation during mitosis.
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DOI：
10.1083/jcb.200504091
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发表时间：
2005-11-07
期刊：
影响因子：
--
通讯作者：
Inagaki M
中科院分区：
文献类型：
--
作者：
Yamaguchi T;Goto H;Yokoyama T;Silljé H;Hanisch A;Uldschmid A;Takai Y;Oguri T;Nigg EA;Inagaki M
在有丝分裂过程中，有几种激酶使最常见的中间丝蛋白波形蛋白磷酸化。Aurora-B和rho激酶通过裂解沟特异性的波形蛋白磷酸化调节波形蛋白丝的分离。Cdk1也能使vimentin从前期到中期磷酸化，但其意义尚不清楚。在这里，我们证明了Plk1和被Cdk1磷酸化的vimentin- ser55之间的直接相互作用，这一事件导致Plk1激活和进一步的vimentin磷酸化。Plk1在体外以1 mol磷酸/mol底物磷酸化vimentin，部分抑制其成丝能力。Plk1诱导vimentin-Ser82的磷酸化，从中期开始升高并维持到有丝分裂结束。这种升高遵循cdk1诱导的vimentin-Ser55磷酸化，并因Plk1缺失而受损。突变分析显示，plk1诱导的vimentin- ser82磷酸化在vimentin细丝分离中起重要作用，与rho激酶和Aurora-B协同作用。综上所述，这些结果表明了一种新的机制，Cdk1不仅通过直接的酶反应，还通过Plk1募集到vimentin来调节有丝分裂的vimentin磷酸化。
Several kinases phosphorylate vimentin, the most common intermediate filament protein, in mitosis. Aurora-B and Rho-kinase regulate vimentin filament separation through the cleavage furrow-specific vimentin phosphorylation. Cdk1 also phosphorylates vimentin from prometaphase to metaphase, but its significance has remained unknown. Here we demonstrated a direct interaction between Plk1 and vimentin-Ser55 phosphorylated by Cdk1, an event that led to Plk1 activation and further vimentin phosphorylation. Plk1 phosphorylated vimentin at ∼1 mol phosphate/mol substrate, which partly inhibited its filament forming ability, in vitro. Plk1 induced the phosphorylation of vimentin-Ser82, which was elevated from metaphase and maintained until the end of mitosis. This elevation followed the Cdk1-induced vimentin-Ser55 phosphorylation, and was impaired by Plk1 depletion. Mutational analyses revealed that Plk1-induced vimentin-Ser82 phosphorylation plays an important role in vimentin filaments segregation, coordinately with Rho-kinase and Aurora-B. Taken together, these results indicated a novel mechanism that Cdk1 regulated mitotic vimentin phosphorylation via not only a direct enzyme reaction but also Plk1 recruitment to vimentin.
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影响因子：
4.8
作者：
Goto, H;Yasui, Y;Inagaki, M
通讯作者：
Inagaki, M
影响因子：
4.8
作者：
Inada, H;Togashi, H;Inagaki, M
通讯作者：
Inagaki, M
DOI：
10.1073/pnas.132269599
发表时间：
2002-06-25
影响因子：
11.1
作者：
Liu, XQ;Erikson, RL
通讯作者：
Erikson, RL
影响因子：
56.9
作者：
Elia, AEH;Cantley, LC;Yaffe, MB
通讯作者：
Yaffe, MB
影响因子：
64.8
作者：
INAGAKI, M;NISHI, Y;SATO, C
通讯作者：
SATO, C