Cdc42 regulate the apoptotic cell death required for planarian epidermal regeneration and homeostasis
Cdc42 regulate the apoptotic cell death required for planarian epidermal regeneration and homeostasis
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Cdc42 调节涡虫表皮再生和稳态所需的细胞凋亡
DOI：
10.1016/j.biocel.2019.05.008
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发表时间：
2019
影响因子：
4
通讯作者：
Zhang Shoutao
中科院分区：
文献类型：
--
作者：
Sun Yujia;Gao Tingting;Li Jiaxin;Zhang Saisai;Hao Zhitai;Tian Qingnan;Zhang Shoutao
Rho GTPases在细胞内通过调节肌动蛋白和微管骨架的组织结构而发挥重要作用。然而，Rho GTP酶在再生过程中整合细胞反应的机制尚未完全阐明。扁形虫体内含有大量成体干细胞，是研究干细胞谱系发育的独特模型。在这里，我们集中在cdc42，这是一个广泛的特征成员在Rho GTP酶。结果表明，Cdc42是维持表皮细胞系所必需的，Cdc42 RNAi可诱导表皮细胞死亡率持续增加，导致成熟表皮细胞丢失，但不影响细胞分裂。我们的研究结果表明cdc42作为一种抑制剂，在真涡虫表皮再生和内环境稳定中阻断过度的凋亡性细胞死亡。
Rho GTPases have been shown previously to play important roles in several cellular processes by regulating the organization of the actin and microtubule cytoskeletons. However, the mechanisms of Rho GTPases that integrate the cellular responses during regeneration have not been thoroughly elucidated. The planarian flatworm, which contains a large number of adult somatic stem cells (neoblasts), is a unique model to study stem cell lineage developmentin vivo. Here, we focus oncdc42, which is an extensively characterized member among Rho GTPases. We found thatcdc42is required for the maintenance of epidermal lineage.Cdc42RNAi induced a sustained increased of cell death and led to a loss of the mature epidermal cells but without affected cell division. Our results indicate thatcdc42function as an inhibitor to block the excessive apoptotic cell death in planarian epidermal regeneration and homeostasis.