Morphological, transcriptional, and metabolic analyses of osmotic-adapted mechanisms of the halophilic Aspergillus montevidensis ZYD4 under hypersaline conditions
Morphological, transcriptional, and metabolic analyses of osmotic-adapted mechanisms of the halophilic Aspergillus montevidensis ZYD4 under hypersaline conditions
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DOI：
10.1007/s00253-019-09705-2
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发表时间：
2019-05-01
影响因子：
5
通讯作者：
Gong, Guoli
中科院分区：
文献类型：
--
作者：
Ding, Xiaowei;Liu, Kaihui;Gong, Guoli
高盐生境中的嗜盐真菌需要多种细胞反应来适应高盐环境。然而，这些适应过程背后的确切机制仍然是鲜为人知的。本研究旨在阐明嗜盐真菌Aspergillus montevidensis ZYD 4在高盐条件下的形态学、转录组学和代谢组学变化。在这些条件下，真菌促进分生孢子的形成和抑制闭囊壳发育。此外，真菌差异表达控制离子转运、氨基酸转运和代谢、可溶性糖积累、脂肪酸β氧化、饱和脂肪酸合成、电子传递和氧化应激耐受性的基因（P < 0.0001）。此外，高盐化菌丝体广泛积累代谢产物，包括氨基酸、可溶性糖、饱和脂肪酸和其他含碳和含氮化合物。添加代谢产物如新橙皮苷、缩二脲、天冬氨酸、丙氨酸、脯氨酸和鸟氨酸显著促进了生长（P
Halophilic fungi in hypersaline habitats require multiple cellular responses for high-salinity adaptation. However, the exact mechanisms behind these adaptation processes remain to be slightly known. The current study is aimed at elucidating the morphological, transcriptomic, and metabolomic changes of the halophilic fungus Aspergillus montevidensis ZYD4 under hypersaline conditions. Under these conditions, the fungus promoted conidia formation and suppressed cleistothecium development. Furthermore, the fungus differentially expressed genes (P < 0.0001) that controlled ion transport, amino acid transport and metabolism, soluble sugar accumulation, fatty acid beta-oxidation, saturated fatty acid synthesis, electron transfer, and oxidative stress tolerance. Additionally, the hypersalinized mycelia widely accumulated metabolites, including amino acids, soluble sugars, saturated fatty acids, and other carbon- and nitrogen-containing compounds. The addition of metabolites-such as neohesperidin, biuret, aspartic acid, alanine, proline, and ornithine-significantly promoted the growth (P