Changes in the pattern of androgen formation in vitro by the baboon fetal adrenal gland at mid- and late gestation.
Changes in the pattern of androgen formation in vitro by the baboon fetal adrenal gland at mid- and late gestation.
复制标题
妊娠中期和晚期狒狒胎儿肾上腺体外雄激素形成模式的变化。
DOI：
10.1095/biolreprod37.5.1192
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发表时间：
1987
影响因子：
3.6
通讯作者：
Albrecht,ED
中科院分区：
文献类型：
--
作者：
Walker,ML;Pepe,GJ;Albrecht,ED
在本研究中，在妊娠中期和晚期获得狒狒胎儿肾上腺细胞，并孵育不同的时间间隔，以同时确定孵育时间长度和发育阶段对肾上腺类固醇生成模式的影响。将细胞用促肾上腺皮质激素（ACTH）处理0至48小时，并测定培养基中脱氢表雄酮（DHA）、DHA硫酸盐（DHAS）和雄烯二酮（Δ4A）的浓度。未经处理或经 ACTH 处理的妊娠中期细胞的 DHA 和 DHAS 分泌在整个 48 小时潜伏期内呈线性增加。然而，在妊娠晚期的胎儿肾上腺孵化器中，DHA 和 DHAS 浓度在 3 小时达到峰值，此后下降，表明分泌到培养基中的 DHA 被该组织进一步代谢。狒狒胎儿肾上腺细胞在妊娠中期形成相似数量的 DHAS 和 DHA，但在足月时形成更多的 DHAS。在妊娠中期的胎儿肾上腺孵化中，DHA 浓度超过 Δ4A 的三倍，这种关系在妊娠后期逆转，可能是由于随着妊娠的进展，3β-羟基类固醇脱氢酶的活性增加。由于 DHA 随着培养时间的推移而下降，也与 DHAS 的伴随下降有关，而 Δ4A 没有变化，因此这些类固醇的形成似乎并不能解释 DHA 的损失。我们的结论是，妊娠中期胎儿肾上腺细胞表现出的雄激素代谢模式与足月时不同。这项研究表明，只有通过同时研究妊娠期两次的类固醇生成并采用多次孵育，胎儿肾上腺雄激素代谢的差异才会显现出来。
In the present study, baboon fetal adrenal cells were obtained at mid- and late gestation and incubated for various intervals to determine simultaneously the effects of length of incubation and stage of development on the pattern of adrenal steroidogenesis. Cells were treated with adrenocorticotropic hormone (ACTH) from 0 to 48 h of incubation, and the concentrations of dehydroepiandrosterone (DHA), DHA-sulfate (DHAS), and androstenedione (Δ4A) were determined in the medium. The secretion of DHA and DHAS by untreated or ACTH-treated cells of midgestation increased linearly throughout the 48-h incubation period. In fetal adrenal incubates of late gestation, however, DHA and DHAS concentrations peaked at 3 h and declined thereafter, suggesting that the DHA secreted into the medium was further metabolized by this tissue. Baboon fetal adrenal cells formed similar amounts of DHAS and DHA at midgestation, but greater quantities of DHAS were formed at term. In fetal adrenal incubates of midgestation, DHA concentrations exceeded those of Δ4A by threefold, a relationship which was reversed at late gestation, probably due to the increase in the activity of 3 β-hydroxy-steroid dehydrogenase with advancing gestation. Because the decline in DHA with time of incubation was also associated with a concomitant decrease in DHAS and no change in Δ4A, it does not appear that formation of these steroids account for the loss of DHA. We conclude that the pattern of androgen metabolism exhibited by fetal adrenal cells obtained at midgestation is different from that at term. This study illustrates that differences in androgen metabolism by the fetal adrenal gland are apparent only by simultaneously investigating steroidogenesis at two times in gestation and employing multiple times of incubation.