STRONG CONSERVATION OF NONCODING SEQUENCES DURING VERTEBRATES EVOLUTION - POTENTIAL INVOLVEMENT IN POSTTRANSCRIPTIONAL REGULATION OF GENE-EXPRESSION
STRONG CONSERVATION OF NONCODING SEQUENCES DURING VERTEBRATES EVOLUTION - POTENTIAL INVOLVEMENT IN POSTTRANSCRIPTIONAL REGULATION OF GENE-EXPRESSION
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DOI：
10.1093/nar/21.10.2315
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发表时间：
1993-05-25
影响因子：
14.9
通讯作者：
GAUTIER, C
中科院分区：
文献类型：
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作者：
DURET, L;DORKELD, F;GAUTIER, C
对 3 亿多年前分歧的不同脊椎动物类别的核苷酸序列进行比较，揭示了基因非编码部分在 100 至 1450 nt 范围内存在高度保守区域 (HCR)，其相似性超过 70%。这种保守性是出乎意料的，因为它比特定调节蛋白的结合所需的保守性更长、更强。 HCR 相对频繁，尤其是在细胞生命必需的基因中。在多基因家族中，保守区域是每种同种型所特有的，并且可能参与其特定表达模式的控制。研究基因内 HCR 分布表明，3' 非编码区的功能限制通常比启动子或内含子强得多。 3'-HCR 特别富含 A + T，并且始终位于基因的转录非翻译区域，这表明它们参与转录后过程。然而，目前对调节 mRNA 输出、定位、翻译或降解的机制的了解不足以解释我们所描述的强大的功能限制。
Comparison of nucleotide sequences from different classes of vertebrates that diverged more than 300 million years ago, revealed the existence of highly conserved regions (HCRs) with more than 70% similarity over 100 to 1450 nt in non-coding parts of genes. Such a conservation is unexpected because it is much longer and stronger than what is necessary for specifying the binding of a regulatory protein. HCRs are relatively frequent, particularly in genes that are essential to cell life. In multigene families, conserved regions are specific of each isotype and are probably involved in the control of their specific pattern of expression. Studying HCRs distribution within genes showed that functional constraints are generally much stronger in 3'-non-coding regions than in promoters or introns. The 3'-HCRs are particularly A + T-rich and are always located in the transcribed untranslated regions of genes, which suggests.that they are involved in post-transcriptional processes. However, current knowledge of mechanisms that regulate mRNA export, localisation, translation, or degradation is not sufficient-to explain the strong functional constraints that we have characterised.