Reduced transforming growth factor-β1 production by mononuclear cells from patients with active chronic idiopathic thrombocytopenic purpura
Reduced transforming growth factor-β1 production by mononuclear cells from patients with active chronic idiopathic thrombocytopenic purpura
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DOI：
10.1046/j.0007-1048.2002.03345.x
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发表时间：
2002-03-01
影响因子：
6.5
通讯作者：
Wadenvik, H
中科院分区：
文献类型：
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作者：
Andersson, PO;Olsson, A;Wadenvik, H
慢性特发性血小板减少性紫癜（ITP）是一种自身免疫性疾病，其中活化的T辅助细胞（Th）和不同的Th细胞因子可能起着重要作用。我们最近报道，慢性ITP患者在缓解有升高的血浆水平的Th 3细胞因子转化生长因子-β 1（TGF-β 1）。可能是免疫抑制的一部分在本研究中，我们发现，在患有活动性疾病的ITP患者[血小板计数（plc）< 50 × 10（9）/l]中，丝裂原刺激的外周血单个核细胞（PBMC）产生TGF-β 1显著减少（444 178 pg/ml; n = 6）与PLC 50-150 x 10（9）/l患者相比（1293 +/- 374 pg/ml：n = 9：P < 0.05），plc > 150 x 10（9）/l的患者（1894 244 pg/ml; n = 12：P < 0.005）和健康对照组（1698 241 pg/ml; n = 10; P < 0.01）。19%的up患者表达血小板诱导的PBMC增殖。令人惊讶。22%的ITP患者的PBMC增殖低于正常范围，即在存在血小板的情况下增殖受到抑制;这6名患者中有活动性疾病。总之。该研究表明，患有活动性疾病的慢性ITP患者PBMC产生的Th 3细胞因子TGF-β 1减少。这一结果进一步支持了活动期慢性ITP与Th 3-应答下调有关的理论。
Chronic idiopathic thrombocytopenic purpura (ITP) is an autoimmune disorder in which activated T-helper (Th) cells and different Th-cell cytokines might play an important role. We have recently reported that chronic ITP patients in remission had elevated plasma levels of the Th3 cytokine transforming growth factor-beta1 (TGF-beta1). possibly as a part of a bystander immune suppression. In the present study we found that, in ITP patients with active disease [platelet count (plc) < 50 x 10(9)/l], mitogen-stimulated peripheral blood mononuclear cells (PBMC) had a significantly reduced production of TGF-beta 1 (444 178 pg/ml; n = 6) compared with patients with plc 50-150 x 10(9)/l (1293 +/- 374 pg/ml: n 9: P < 0.05), patients with plc > 150 x 10(9)/l (1894 244 pg/ml; n = 12: P < 0.005) and healthy controls (1698 241 pg/ml; n = 10; P < 0.01). Nineteen per cent of up patients expressed a platelet-induced PBMC proliferation. Surprisingly. 22% of the ITP patients had a PBMC proliferation below the normal range, i.e. a suppressed proliferation in the presence of platelets; live of these six patients had active disease. In summary. this study demonstrated that chronic ITP patients with active disease had reduced PBMC production of the Th3 cytokine TGF-beta1. This result gives further support to the theory that chronic ITP in active phase is associated with it downregulatcd Th3-response.