Modulating cellular cytotoxicity and phototoxicity of fluorescent organic salts through counterion pairing
Modulating cellular cytotoxicity and phototoxicity of fluorescent organic salts through counterion pairing
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DOI：
10.1038/s41598-019-51593-z
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发表时间：
2019-10-25
影响因子：
4.6
通讯作者：
Lunt, Sophia Y.
中科院分区：
文献类型：
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作者：
Broadwater, Deanna;Bates, Matthew;Lunt, Sophia Y.
光激活治疗诊断学为疾病诊断、图像引导手术和特定部位的个性化治疗提供了有希望的机会。然而，目前的荧光染料受到低亮度、高细胞毒性、差的组织渗透性和不希望的副作用的限制。为了克服这些限制，我们展示了一个光电调谐平台，它允许独立控制的光学性质从荧光有机盐的电子性质。这是通过有机盐的阳离子-阴离子配对来实现的，有机盐可以调节前沿分子轨道而不影响带隙。光电调谐能够通过选择性产生控制细胞活力的线粒体活性氧物质来解耦控制荧光有机盐的细胞毒性和光毒性。我们表明，通过互补配对，有机盐纳米粒子可以被调整为无毒的增强成像，或光毒性的改善光动力疗法。
Light-activated theranostics offer promising opportunities for disease diagnosis, image-guided surgery, and site-specific personalized therapy. However, current fluorescent dyes are limited by low brightness, high cytotoxicity, poor tissue penetration, and unwanted side effects. To overcome these limitations, we demonstrate a platform for optoelectronic tuning, which allows independent control of the optical properties from the electronic properties of fluorescent organic salts. This is achieved through cationanion pairing of organic salts that can modulate the frontier molecular orbital without impacting the bandgap. Optoelectronic tuning enables decoupled control over the cytotoxicity and phototoxicity of fluorescent organic salts by selective generation of mitochondrial reactive oxygen species that control cell viability. We show that through counterion pairing, organic salt nanoparticles can be tuned to be either nontoxic for enhanced imaging, or phototoxic for improved photodynamic therapy.