Mutation of ZmWRKY86 confers enhanced salt stress tolerance in maize
Mutation of ZmWRKY86 confers enhanced salt stress tolerance in maize
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ZmWRKY86 突变增强玉米的盐胁迫耐受性
DOI：
10.1016/j.plaphy.2021.09.010
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发表时间：
2021-09-14
影响因子：
6.5
通讯作者：
Cai, Ronghao
中科院分区：
文献类型：
--
作者：
Fang, Xiu;Li, Wei;Cai, Ronghao
WRKY蛋白是植物中最大的转录因子家族之一，在植物的生长发育、防御调节和逆境应答中起着重要作用。本研究从玉米（Zea mays L.）中克隆了编码WRKY转录因子的ZmWRKY 86。ZmWRKY 86的表达在盐胁迫下上调。ZmWRKY 86是一种核蛋白，在酵母中没有转录激活能力。ZmWRKY 86可与W-box（TTGACC）特异性结合，这一点通过电泳迁移率变动分析（EMSA）和双LUC实验得到证实。与对照相比，wrky 86突变体在盐胁迫下的存活率、过氧化氢酶活性和K+含量显著提高，丙二醛积累量、相对漏电率和Na+含量显著降低，表现出较强的耐盐性。转录组和实时荧光定量PCR分析表明，ZmWRKY 86突变导致玉米逆境相关基因的表达发生显著变化。进一步的分析表明ZmWRKY 86与两个盐胁迫相关基因Zm 00001 d 020840和Zm 00001 d 046813的启动子直接相互作用。综上所述，我们鉴定了一个WRKY转录因子ZmWRKY 86，其通过调控胁迫相关基因的表达参与盐胁迫调控。
As one of the largest families of transcription factors in plants, the WRKY proteins play crucial roles in plant growth and development, defense regulation and stress responses. In this research, ZmWRKY86 encoding a WRKY transcription factor was cloned from maize (Zea mays L.). ZmWRKY86 expression was up-regulated by salt stress. ZmWRKY86 is a nuclear protein and has no transcriptional activation ability in yeast. ZmWRKY86 can specifically bind to W-box (TTGACC), which was confirmed by electrophoretic mobility shift assay (EMSA) and dual-LUC experiments. As compared with control, the wrky86 mutants showed enhanced plant tolerance to salt stress with higher survival rate, catalase activity and K+ content, but lower malondialdehyde accumulation, relative electrical leakage level and Na+ content under salt-stress condition. Transcriptome and quantitative realtime PCR analyses indicated that the mutation of ZmWRKY86 led to significant changes in the expression of stress-related genes in maize. Further assays showed that ZmWRKY86 directly interacted with the promoter of two salt stress-related genes Zm00001d020840 and Zm00001d046813. In summary, we identified a WRKY transcription factor ZmWRKY86 that participates in salt stress regulation through controlling the expression of stress-related genes.