Solid‐phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies

Solid‐phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies
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固相微量滴定放射免疫法检测急性非细菌性流行性胃肠炎病毒诺瓦克株及其抗体

DOI:
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发表时间:
1978
影响因子:
12.7
通讯作者:
A. Kapikian
A. Kapikian
中科院分区:
医学3区
文献类型:
--
作者:
H. Greenberg;R. Wyatt;J. Valdesuso;A. Kalica;W. London;R. Chanock;A. Kapikian

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介绍了用于检测诺瓦克抗原及其抗体的微滴定固相放射免疫分析方法的研究进展。这些测试很容易进行,而且敏感和特异。抗原检测可用于粗粪、滤液和悬浮液。这两种检测至少与免疫电子显微镜一样灵敏,但比免疫粘附试验更灵敏。
The development of microtiter solid‐phase radioimmunoassays for the detection of Norwalk antigen and its antibody is described. The tests are simple to perform and are sensitive and specific. The test for antigen can be used on crude stool filtrates and suspensions. Both tests are at least as sensitive as immune electron microscopy and more sensitive than immune adherence assay.