Adult human hematopoietic cells provide functional hemangioblast activity
Adult human hematopoietic cells provide functional hemangioblast activity
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DOI：
10.1182/blood-2003-06-2101
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发表时间：
2004-01-01
期刊：
影响因子：
20.3
通讯作者：
Scott, EW
中科院分区：
文献类型：
--
作者：
Cogle, CR;Wainman, DA;Scott, EW
鼠成体造血干细胞能够作为成血管细胞发挥功能，有助于血液重建和响应缺血性损伤的血管修复。我们开发了一种新的小鼠异种移植模型的视网膜新生血管测试人类造血细胞的可塑性。免疫功能低下的非肥胖糖尿病（NOD）/scid小鼠接受清髓性预处理和人CD 34（+）脐带血移植。在建立多系重建后，诱导视网膜缺血以促进新生血管形成。我们的研究结果表明，人类视网膜新生血管，从而揭示了人类造血细胞的功能成血管细胞的活动。
The murine adult hematopoietic stem cell is able to function as a hemangioblast, contributing both to blood reconstitution and to blood vessel repair in response to ischemic injury. We developed a novel mouse xenotransplantation model of retinal neovascularization to test human hematopoietic cell plasticity. Immunocompromised nonobese diabetic (NOD)/scid mice underwent myeloablative conditioning and transplantation with human CD34(+) umbilical cord blood. After multilineage reconstitution was established, retinal ischemia was induced to promote neovascularization. Our results demonstrate human retinal neovascularization, thus revealing the functional hemangioblast activity of human hematopoietic cells.