Bright, near infrared emitting PLGA-PEG dye-doped CN-PPV nanoparticles for imaging applications
Bright, near infrared emitting PLGA-PEG dye-doped CN-PPV nanoparticles for imaging applications
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DOI：
10.1039/c6ra25004a
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Dailey, Lea Ann
中科院分区：
文献类型：
--
作者：
Kemal, Evren;Abelha, Thais Fedatto;Dailey, Lea Ann
共轭聚合物作为临床诊断应用的光学成像探针受到关注。然而，临床转化不仅需要出色的光学性能，还需要既定的安全性和可扩展的可制造性。考虑到这些因素，设计了一种自组装纳米粒子系统，利用两亲性二嵌段共聚物PLGA-PEG来封装发红光的共轭聚合物CN-PPV。减少 CN-PPV 量（50% 至 5% w/w）的封装导致纳米颗粒尺寸减小和光学性能提高（量子产率% 类似于 40%）。所有系统在 37 摄氏度下在 60 天内都是胶体和光学稳定的。然后使用优化的系统封装少量（0.5-0.8% w/w）的小分子近红外染料 NIR680 和 NIR720，生成发射峰偏移 > 700 nm 的系统。与本研究中测试的其他系统相比，含有 5% CN-PPV 和 0.5% NIR720 的优化 PLGA-PEG 胶束显示出增强的特性，例如高产物产率 (> 90%)、720 nm 处的窄发射峰、45% 的高量子产率、小流体动力学直径（类似于 104 nm）以及增强的细胞相容性特征，即细胞活力和细胞活性没有降低。在较高浓度下线粒体活性的损害可以忽略不计。
Conjugated polymers are of interest as optical imaging probes for clinical diagnostic applications. However, clinical translation requires not only an excellent optical performance, but also an established safety profile and scalable manufacturability. Taking these factors into account, a self-assembling nanoparticle system was designed utilising the amphiphilic diblock copolymer, PLGA-PEG, to encapsulate the red-emitting conjugated polymer, CN-PPV. Encapsulation of decreasing amounts of CN-PPV (50% to 5% w/w) resulted in a decrease in nanoparticle size and an increase in optical performance (quantum yield% similar to 40%). All systems were colloidally and optically stable over 60 days at 37 degrees C. Optimized systems were then used to encapsulate small amounts (0.5-0.8% w/w) of small molecule near-infrared dyes, NIR680 and NIR720, generating systems with shifted emission peaks > 700 nm. Optimised PLGA-PEG micelles containing 5% CN-PPV and 0.5% NIR720 showed enhanced characteristics, such as a high product yield (> 90%), a narrow emission peak at 720 nm, a high quantum yield of 45%, a small hydrodynamic diameter (similar to 104 nm), and an enhanced cytocompatibility profile compared to other systems tested in this study, i.e. no reduction in cell viability and negligible impairment of mitochondrial acitivity at higher concentrations.