Involvement of activin a receptor type 1 (ACVR1) in the pathogenesis of primary focal hyperhidrosis
Involvement of activin a receptor type 1 (ACVR1) in the pathogenesis of primary focal hyperhidrosis
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激活素 A 受体 1 型 (ACVR1) 参与原发性局灶性多汗症的发病机制
DOI：
10.1016/j.bbrc.2020.05.052
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发表时间：
2020-07-23
影响因子：
3.1
通讯作者：
Kang, Ming-Qiang
中科院分区：
文献类型：
--
作者：
Lin, Jian-Bo;Chen, Jian-Feng;Kang, Ming-Qiang
原发性局灶性多汗症（PFH）的发病机制尚不清楚。 PFH被认为是一种遗传性疾病。激活素 A 受体 1 型 (ACVR1) 是否参与 PFH 的发病机制尚不清楚。本研究采用蛋白质印迹法和免疫荧光法检测PAH患者汗腺中ACVR1的表达情况。从原发性腋窝多汗症（PAH）患者获得的原代汗腺细胞用 acvr1 载体转染。 acvr1载体转染后检测腺细胞的细胞增殖、凋亡和细胞周期。检测水通道蛋白5（AQP5）和Na:K:2Cl协同转运蛋白1（NKCC1/SLC12A2）的mRNA和蛋白表达。我们的数据显示PAH患者腋窝汗腺组织中ACVR1的表达量显着高于正常对照组。在从 PAH 患者获得的腺细胞中进一步研究了 ACVR1 的功能。与NC组相比，ACVR1过表达显着促进汗腺细胞增殖，抑制汗腺细胞凋亡。同时，ACVR1过表达显着降低了G0/G1和G2/M期细胞的百分比，并增加了S期细胞的百分比。此外，ACVR1过表达显着促进AQP5和NKCC1在mRNA和蛋白水平上的表达。总之，ACVR1的表达与PFH相关，ACVR1的过表达可以通过促进AQP5和NKCC1的表达来促进汗腺细胞的增殖并抑制细胞凋亡。 (C) 2020 Elsevier Inc. 保留所有权利。
The pathogenesis of primary focal hyperhidrosis (PFH) is still not clear. PFH is thought to be a genetic disease. Whether activin A receptor type 1 (ACVR1) is involved in the pathogenesis of PFH is unknown. In this study, the expression of ACVR1 in sweat glands of patients with PAH was detected by western blot and immunofluorescence. The primary sweat gland cells obtained from primary axillary hyperhidrosis (PAH) patients were transfected with acvr1 vector. Cell proliferation, apoptosis and cell cycling of gland cells were measured after transfection with acvr1 vector. The mRNA and protein expression of aquaporin 5 (AQP5) and Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) were detected. Our data showed that ACVR1 expression in axillary sweat gland tissue of PAH patients was significantly higher than that of normal control group. The function of ACVR1 was further investigated in the gland cells obtained from PAH patients. Compared with NC group, ACVR1 overexpression significantly promoted the proliferation of sweat gland cells and inhibited the apoptosis of sweat gland cells. Meanwhile, ACVR1 overexpression significantly reduced the percentage of cells in G0/G1 and G2/M phases, and increased the percentage of cells in S phase. In addition, ACVR1 overexpression significantly promoted the expression of AQP5 and NKCC1 at both mRNA and protein levels. Together, ACVR1 expression is related to PFH and ACVR1 overexpression can promote the proliferation of sweat gland cells and inhibit apoptosis by promoting the expression of AQP5 and NKCC1. (C) 2020 Elsevier Inc. All rights reserved.