Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index
Novel association between sperm reactive oxygen species production, sperm morphological defects, and the sperm deformity index
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DOI：
10.1016/j.fertnstert.2003.06.026
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发表时间：
2004-02-01
影响因子：
6.7
通讯作者：
Agarwal, A
中科院分区：
文献类型：
--
作者：
Aziz, N;Saleh, RA;Agarwal, A
设计：一项前瞻性的临床研究。地点：男性不育门诊，格利克曼泌尿研究所，克利夫兰临床基金会，俄亥俄州克利夫兰，克利夫兰临床基金会，英国利物浦妇女医院生殖医学部，患者(S)：不育男性39例，健康生育捐献者13例(对照组)。干预(S)：标准精液分析，精液白细胞浓度，精子形态评估，精子ROS生成量测量。主要结果衡量(S)：精子ROS生成水平，结果：精子ROS的产生与形态正常和边缘形态的精子比例呈显著负相关(S)。活性氧的产生与精子头部无定形、顶体受损、中段缺陷、细胞质小滴、尾部缺陷和SDI评分呈正相关。Logistic回归分析确定了一个包括SDI和精子活动率的双变量模型，该模型正确地识别了84%的精液ROS高和85%的精液ROS低的个体。该模型的总体准确率为85%。结论：标准精液分析评估精子活力、精子形态和SDI评分，在没有精液ROS水平测量设施的不育诊所中，是识别精液ROS水平高的不育男性的有用工具。(C)2004年，由美国生殖医学会提供。
Objective: To examine the relationship between sperm reactive oxygen species (ROS) production and sperm morphology in a group of infertile men and healthy fertile donors.Design: A prospective clinical study.Setting: Male infertility clinic, Glickman Urological Institute, The Cleveland Clinic Foundation, Cleveland, Ohio, and the Reproductive Medicine Unit, Liverpool Women's Hospital, United Kingdom Patient(s): Thirty-nine infertile men and 13 healthy fertile donors (control).Intervention(s): Standard semen analysis, seminal leukocyte concentration, assessment of sperm morphology, and measurement of sperm ROS production.Main Outcome Measure(s): Levels of sperm ROS production, percentages of different sperm morphological abnormalities, and the sperm deformity index (SDI) scores.Result(s): A significant negative correlation was observed between sperm ROS production and the proportion of sperm with normal morphology and borderline morphology. Reactive oxygen species production was positively correlated with the proportion of sperm with amorphous heads, damaged acrosomes, midpiece defects, cytoplasmic droplets, tail defects, and SDI scores. Logistic regression analysis identified a two-variable model including SDI and percentage sperm motility, which correctly identified 84% of individuals with high seminal ROS and 85% of individuals with low seminal ROS. The model had an overall accuracy of 85%.Conclusion(s): The standard semen analysis to assess sperm motility, sperm morphology, and the SDI scores is a useful tool in identifying infertile men with high seminal ROS in infertility clinics where facilities for measuring levels of seminal ROS are not available. (C)2004 by American Society for Reproductive Medicine.