Haloarchaeal diversity in 23, 121 and 419 MYA salts
Haloarchaeal diversity in 23, 121 and 419 MYA salts
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DOI：
10.1111/j.1472-4669.2009.00218.x
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发表时间：
2009-12
期刊：
影响因子：
3.7
通讯作者：
Jong Soo Park;R. Vreeland;B. Cho;T. Lowenstein;M. Timofeeff;W. Rosenzweig
中科院分区：
文献类型：
--
作者：
Jong Soo Park;R. Vreeland;B. Cho;T. Lowenstein;M. Timofeeff;W. Rosenzweig
从不同地质年龄（23，121，419百万年，MYA）的表面灭菌盐中提取DNA，以研究盐古菌的多样性。在2300万年前的盐中只发现了盐囊菌和盐红菌DNA。较老的晶体含有未分类的群体和盐杆菌。较老的晶体在16S rRNA V2区域内产生了独特的55 bp插入片段。V2区的二级结构完全不同于现代环境中的盐古菌。DNA表明，未知的盐古菌和盐杆菌是古代高盐环境的关键组成部分。Halorubrum和Haloarcula似乎是相对现代的高盐生境中的优势群体。
DNA was extracted from surface‐sterilized salt of different geological ages (23, 121, 419 million years of age, MYA) to investigate haloarchaeal diversity. Only Haloarcula and Halorubrum DNA was found in 23 MYA salt. Older crystals contained unclassified groups and Halobacterium. The older crystals yielded a unique 55‐bp insert within the 16S rRNA V2 region. The secondary structure of the V2 region completely differed from that in haloarchaea of modern environments. The DNA demonstrates that unknown haloarchaea and the Halobacterium were key components in ancient hypersaline environments. Halorubrum and Haloarcula appear to be a dominant group in relatively modern hypersaline habitats.