IL-4 addition during differentiation of CD34 progenitors delays maturation of dendritic cells while promoting their survival
IL-4 addition during differentiation of CD34 progenitors delays maturation of dendritic cells while promoting their survival
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DOI：
10.1016/s0171-9335(98)80124-6
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发表时间：
1998-03-01
影响因子：
6.6
通讯作者：
Vincent, C
中科院分区：
文献类型：
--
作者：
Rougier, N;Schmitt, D;Vincent, C
Dendritic cells (DC) are the most effective cells for antigen presentation in primary immune responses. Human cord blood CD34(+) progenitors cultured in the presence of GM-CSF and TNF alpha generate a heterogeneous population of DC including Langerhans-like DC (LLDC) and monocytes. We describe here that IL-4 exerts different effects in such culture according to the cells considered. Thus, IL-4 favors DC components at the expense of monocytic development, and permits long-time persistence of DC which can be maintained up to one month in culture.These results show an IL-4-dependent inhibition of proliferation and emergence of CD14(+) cells. Notably, however, IL-4 also acts on the DC precursors. Thus, IL-4 enhances survival and delays maturation of LLDC from CD1a(+) CD14(-) precursors. In addition, IL-4 also favors orientation of CD14(+) CD1a(-) DC/monocyte precursors towards dermal-type CD1a(+) DC. DC recovered from IL-4 treated cultures display reduced allostimulatory capacity, but this function is restored upon IL-4 weaning. Finally, a short (48h) IL-4 pulse is sufficient to favor DC development.The present study demonstrates that IL-4 positively regulates DC development at several levels on distinct precursor cells.
