A Papertronic Sensing System for Rapid Visual Screening of Bacterial Electrogenicity
A Papertronic Sensing System for Rapid Visual Screening of Bacterial Electrogenicity
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DOI：
10.1088/1742-6596/1407/1/012094
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发表时间：
2019-11
期刊：
影响因子：
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通讯作者：
Mehdi Tahernia;M. Mohammadifar;Seokheun Choi
中科院分区：
文献类型：
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作者：
Mehdi Tahernia;M. Mohammadifar;Seokheun Choi
在这项工作中，我们报告了一个papertronic传感系统的能力，实现简单，快速，灵敏的表征细菌产电性从一个单一的培养液滴。纸被用作装置基底，其固有地产生用于微生物液体样品的容易、快速、灵敏和潜在高通量控制的有利条件。通过一个创新的微型设备结构和一个简单的晶体管放大器电路直接集成到一个单一的纸张基板，一个强大的传感阵列的构建，导致从微升样品体积的细菌产电性的快速和灵敏的表征。微生物电流产生通过晶体管放大，晶体管向4宽LED电路板指示条提供电力，用于用肉眼直接视觉读出。根据细菌的产电性，改变LED强度。我们使用两种已知的细菌产电菌（野生型S。oneidensis和铜绿假单胞菌）和假设驱动的遗传修饰的铜绿假单胞菌突变株。
In this work, we report a papertronic sensing system with the ability to achieve easy, rapid, and sensitive characterization of bacterial electrogenicity from a single drop of culture. Paper was used as a device substrate that inherently produces favorable conditions for easy, rapid, and sensitive and potentially high-throughput controlling of a microbial liquid sample. Through an innovative microscale device structure and a simple transistor amplifier circuit directly integrated into a single sheet of paper substrate, a powerful sensing array was constructed, resulting in the rapid and sensitive characterization of bacterial electrogenicity from a microliter sample volume. The microbial current generations were amplified by the transistor providing power to a 4-wide LED circuit board indicator bar for the direct visual readout with the naked eyes. Depending on bacterial electrogenicity, the LED intensity was changed. We validated the effectiveness of the sensor using two known bacterial electrogens (wild-type S. oneidensis and P. aeruginosa) and hypothesis-driven genetically modified P. aeruginosa mutant strains.