Pulsing Influences Photoradiation Outcomes in Cell Culture
Pulsing Influences Photoradiation Outcomes in Cell Culture
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DOI：
10.1002/lsm.20740
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发表时间：
2009-03-01
影响因子：
2.4
通讯作者：
Lanzafame, Raymond J.
中科院分区：
文献类型：
--
作者：
Brondon, Philip;Stadler, Istvan;Lanzafame, Raymond J.
背景和目的：皮肤色素沉着会对光疗结果产生不利影响。已经提出递送脉冲光作为增强功效的手段。合适的脉冲频率仍然是不确定的，通常根据经验选择。这项研究是为了确定是否脉冲光传递减轻黑色素对光调节的过滤作用在vitro.Study设计和方法：人HEP-2细胞培养在完全DMEM培养基。通过0.025%黑色素过滤器在670 nm（5.0 J/cm（2）/治疗/24小时）下以不同脉冲速率进行光辐射72小时。A组不接受光照处理。B组不进行脉冲治疗。C、D、E、F和G组接受6、18、36、100和600 Hz的治疗。结果：100 Hz刺激48和72 h后细胞增殖最明显（n = 41，P <0.05），100 Hz刺激48和72 h后细胞增殖最明显（n = 41，P < 0.05）。在600 Hz时刺激最大氧化爆发（n = 4，P
Background and Objectives: Skin pigmentation can adversely affect phototherapy outcomes. Delivering pulsed light has been suggested as a means of enhancing efficacy. Suitable pulse frequencies remain indeterminate, often being selected empirically. This study was undertaken to determine whether pulsed light delivery mitigates the filtering effect of melanin pigment on photomodulation in vitro.Study Design and Methods: Human HEP-2 cells were Cultured in complete DMEM media. Photoradiation was delivered through 0.025% melanin filters at 670 nm (5.0 J/cm(2)/treatment/24 hours) for 72 hours at different pulse rates. Group A received no light treatment. Group B received treatments without pulsing. Groups C, D, E, F, and G received treatments at 6, 18, 36, 100, and 600 Hz. Cell proliferation was assessed by MTT assay and oxidative burst was measured using the 2.7 dichloro-fluorescein-diacetate assay.Results: Cell proliferation was maximally stimulated at 100 Hz at 48 and 72 hours (n = 41 P < 0.05). Oxidative burst was maximally stimulated at 600 Hz (n = 4, P