Bioinformatics meets proteomics--bridging the gap between mass spectrometry data analysis and cell biology.
Bioinformatics meets proteomics--bridging the gap between mass spectrometry data analysis and cell biology.
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DOI：
10.1142/s021972000300023x
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发表时间：
2003-04-01
影响因子：
1
通讯作者：
Thibault, P
中科院分区：
文献类型：
--
作者：
Kearney, P;Thibault, P
蛋白质组学研究计划通常包括鉴定任何给定细胞的蛋白质含量、它们的同种型、剪接变体、翻译后修饰、相互作用伴侣和不同条件下的高阶复合物。由于蛋白质组的高度复杂性和相应蛋白质的低丰度，这些研究提出了重大的分析挑战，这往往需要高度灵敏和解析技术。质谱技术在蛋白质组学中起着重要的作用，已成为分子和细胞生物学中不可或缺的工具。然而，鉴于需要破译的信息的复杂性、定量分析的准确性和动态范围、适当的生物信息学软件的可用性以及庞大的数据文件，质谱数据的分析可能是一项艰巨的任务。在过去的十年里，基于质谱的蛋白质组学取得了重大的技术进步，与生物信息学的协同作用对于实现生物发现计划的期望至关重要。我们在这里提出的质谱和生物信息学的技术能力，挖掘细胞蛋白质组的背景下，发现计划，旨在微量蛋白质的鉴定和表达微克量的蛋白质提取物从人体组织。
Proteomics research programs typically comprise the identification of protein content of any given cell, their isoforms, splice variants, post-translational modifications, interacting partners and higher-order complexes under different conditions. These studies present significant analytical challenges owing to the high proteome complexity and the low abundance of the corresponding proteins, which often requires highly sensitive and resolving techniques. Mass spectrometry plays an important role in proteomics and has become an indispensable tool for molecular and cellular biology. However, the analysis of mass spectrometry data can be a daunting task in view of the complexity of the information to decipher, the accuracy and dynamic range of quantitative analysis, the availability of appropriate bioinformatics software and the overwhelming size of data files. The past ten years have witnessed significant technological advances in mass spectrometry-based proteomics and synergy with bioinformatics is vital to fulfill the expectations of biological discovery programs. We present here the technological capabilities of mass spectrometry and bioinformatics for mining the cellular proteome in the context of discovery programs aimed at trace-level protein identification and expression from microgram amounts of protein extracts from human tissues.