Differential immunologic reactivity and processing of glycoproteins gA and gB of herpes simplex virus types 1 and 2 made in Vero and HEp-2 cells.
Differential immunologic reactivity and processing of glycoproteins gA and gB of herpes simplex virus types 1 and 2 made in Vero and HEp-2 cells.
复制标题
Vero 和 HEp-2 细胞中 1 型和 2 型单纯疱疹病毒糖蛋白 gA 和 gB 的差异免疫反应和加工。
DOI：
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发表时间：
1981
影响因子：
11.1
通讯作者：
B. Roizman
中科院分区：
文献类型：
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作者：
L. Pereira;D. Dondero;B. Norrild;B. Roizman
单纯疱疹病毒1型和2型（分别为HSV-1和HSV-2）指定五种主要糖蛋白，命名为gA、gB、gC、gD和gE。以前的研究表明，gA和gB不同的电泳迁移率，但不是在与抗血清制备这些糖蛋白。此外，HSV-1的gA和gB在血清学试验中与HSV-2的相应糖蛋白发生交叉反应。在本文中，我们报告的反应性gA和gB的HSV-1和HSV-2与24个独立衍生的单克隆抗体与这些抗原反应。结果表明：（1）在HEp-2细胞中制备的HSV-1和HSV-2糖蛋白gA和gB的电泳迁移率明显低于在Vero细胞中制备的。(ii)所有单克隆抗体均沉淀感染的HEp-2细胞中产生的gA和gB。其中包括12种中和病毒的单克隆抗体和12种没有。(iii)在HEp-2细胞中产生的HSV-2糖蛋白gA和gB含有类型特异性结构域。然而，针对这些结构域的一个克隆产生的单克隆抗体不与Vero细胞中产生的糖蛋白反应。(iv)感染的Vero细胞的裂解物含有三种低分子量多肽，其也与针对糖蛋白gA和gB的单克隆抗体反应。这些多肽是病毒特异性的，因为HSV-1特异性的那些多肽在电泳迁移率上不同于HSV-2特异性的那些多肽。这些多肽在HEp-2细胞的裂解物中不存在。
Herpes simplex virus types 1 and 2 (HSV-1 and HSV-2, respectively) specify five major glycoproteins designated as gA, gB, gC, gD, and gE. Previous studies have shown that gA and gB differ in electrophoretic mobility but not in reactivity with antisera prepared to each of these glycoproteins. Moreover, gA and gB of HSV-1 crossreact in serologic tests with the corresponding glycoproteins of HSV-2. In this paper, we report on the reactivities of gA and gB of HSV-1 and HSV-2 with 24 independently derived monoclonal antibodies reactive with these antigens. Our results show the following: (i) Electrophoretic mobilities of HSV-1 and HSV-2 glycoproteins gA and gB made in HEp-2 cells are significantly less than those made in Vero cells. (ii) All monoclonal antibodies precipitated both gA and gB made in infected HEp-2 cells. These include 12 monoclonal antibodies that neutralized virus and 12 that did not. (iii) HSV-2 glycoproteins gA and gB made in HEp-2 cells contain type-specific domains. However, monoclonal antibodies produced by one clone directed to these domains did not react with glycoproteins made in Vero cells. (iv) Lysates of infected Vero cells contain three lower molecular weight polypeptides that also reacted with monoclonal antibodies directed to glycoproteins gA and gB. These polypeptides are virus specific inasmuch as those specified by HSV-1 differ in electrophoretic mobilities from those specified by HSV-2. these polypeptides are absent in lysates of HEp-2 cells.