A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture
A continuous electrical cell lysis device using a low dc voltage for a cell transport and rupture
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DOI：
10.1016/j.snb.2006.11.054
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发表时间：
2007-06-10
影响因子：
8.4
通讯作者：
Cho, Young-Ho
中科院分区：
文献类型：
--
作者：
Lee, Dong Woo;Cho, Young-Ho
我们提出了一种连续和低电压的细胞裂解装置，其中通道的宽度和长度改变以产生用于细胞裂解的集中的高电场强度和用于样品运输的低电场强度。先前的细胞裂解装置通过将交流电压施加到电极之间的微间隙来获得用于细胞裂解的高电场强度，并且需要额外的泵或阀用于样品输送。然而，当我们改变连接到直流电压的一对外部电极之间的通道的宽度和长度时，我们产生用于细胞裂解的高电场强度和用于电渗流的低电场强度。因此，本装置执行连续的细胞裂解和样品输送，而不需要额外的流动源或制造用于微间隙的电极的额外过程。实验研究的特点是一个孔，其宽度和长度比微通道的宽度和长度窄20倍，短175倍。在50 V的工作电压下，本装置在孔处产生1.2 kV/cm的高电场强度，以100%的红细胞溶解率破坏细胞，在微通道处产生60 V/cm的低电场强度，以产生30 +/-9 μ m/s的电渗流。总之，本装置能够在低电压下连续自泵细胞裂解;因此，它适合于微全分析系统或芯片实验室的样品预处理组件。(c)2006 Elsevier B. V.保留所有权利。
We present a continuous and low voltage cell lysis device in which a width and length of a channel change to generate focused the high electric field strength for cell lysis and the low electric field strength for a transport of samples. The previous cell lysis devices acquire the high electric field strength for a cell lysis by applying an ac voltage to a micro-gap between electrodes and require additional pumps or valves for a sample transport. However, when we change the width and length of the channel between a pair of external electrodes attached to a dc voltage, we generate both the high electric field strength for a cell lysis and the low electric field strength for an electroosmotic flow. The present device therefore performs continuous cell lysis and a sample transport without needing either an additional flow source or an additional process fabricating the electrodes for the micro-gap. The experimental study features an orifice whose width and length is 20 times narrower and 175 times shorter than the width and length of a microchannel. With an operational voltage of 50 V, the present device generates high electric field strength of 1.2 kV/cm at the orifice to disrupt cells with 100% lysis rate of red blood cells and low electric field strength of 60 V/cm at the microchannel to generate an electroosmotic flow of 30 +/- 9 mu m/s. In conclusion, the present device is capable of continuous self-pumping cell lysis at a low voltage; thus, it is suitable for a sample pretreatment component of a micro total analysis system or lab-on-a-chip. (c) 2006 Elsevier B.V. All rights reserved.