DROP: Molecular voucher database for identification of Drosophila parasitoids.
DROP: Molecular voucher database for identification of Drosophila parasitoids.
复制标题
DROP：用于鉴定果蝇寄生蜂的分子凭证数据库。
DOI：
10.1111/1755-0998.13435
复制
发表时间：
2021
影响因子：
7.7
通讯作者：
Lue CH
中科院分区：
文献类型：
--
作者：
Lue CH
分子鉴定越来越多地用于加速生物多样性调查和实验室实验。然而，由于缺乏专家鉴定的测序凭证标本，许多生物群无法使用标准数据库（如GenBank或BOLD）可靠地鉴定。有时候有大量的序列可用，但有太多的错误，以允许识别。在这里，我们解决这个问题的寄生虫ofDrosophilaby介绍了一个策划的开放获取分子参考数据库，DROP（果蝇寄生虫）。识别果蝇寄生虫具有挑战性，并且在从分子机制到食物网的研究以及对Suzukii果蝇的生物控制中实现该模型系统的全部潜力方面构成了主要障碍。在DROP中，遗传数据与凭证标本相关联，在可能的情况下，凭证标本由分类学家鉴定，并通过与原始类型材料的直接比较进行审查。为了启动DROP，我们策划了154个实验室菌株，856个凭证，554个DNA序列，16个基因组，14个转录组和6个蛋白质组，来自183个操作分类单位（OTU）：114个描述的果蝇寄生虫物种和69个临时物种。我们发现物种丰富度ofDrosophilaparasitoids被严重低估，并提供了一个更新的分类目录的社会。DROP提供了准确的分子鉴定，并改善了个体研究之间的交叉引用，我们希望这将促进对这个多样化和迷人的模型系统的研究。我们的努力也应该作为一个例子，研究人员面临类似的分子鉴定问题，在其他群体的生物。
Molecular identification is increasingly used to speed up biodiversity surveys and laboratory experiments. However, many groups of organisms cannot be reliably identified using standard databases such as GenBank or BOLD due to lack of sequenced voucher specimens identified by experts. Sometimes a large number of sequences are available, but with too many errors to allow identification. Here, we address this problem for parasitoids ofDrosophilaby introducing a curated open‐access molecular reference database, DROP (Drosophilaparasitoids). IdentifyingDrosophilaparasitoids is challenging and poses a major impediment to realize the full potential of this model system in studies ranging from molecular mechanisms to food webs, and in biological control ofDrosophila suzukii. In DROP, genetic data are linked to voucher specimens and, where possible, the voucher specimens are identified by taxonomists and vetted through direct comparison with primary type material. To initiate DROP, we curated 154 laboratory strains, 856 vouchers, 554 DNA sequences, 16 genomes, 14 transcriptomes, and six proteomes drawn from a total of 183 operational taxonomic units (OTUs): 114 describedDrosophilaparasitoid species and 69 provisional species. We found species richness ofDrosophilaparasitoids to be heavily underestimated and provide an updated taxonomic catalogue for the community. DROP offers accurate molecular identification and improves cross‐referencing between individual studies that we hope will catalyse research on this diverse and fascinating model system. Our effort should also serve as an example for researchers facing similar molecular identification problems in other groups of organisms.