Lewisx-containing oligosaccharide attenuates schistosome egg antigen-induced immune depression in human schistosomiasis
Lewisx-containing oligosaccharide attenuates schistosome egg antigen-induced immune depression in human schistosomiasis
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DOI：
10.1016/s0198-8859(99)00136-6
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发表时间：
2000-03-01
期刊：
影响因子：
2.7
通讯作者：
Harn, DA
中科院分区：
文献类型：
--
作者：
Velupillai, P;dos Reis, EA;Harn, DA
对含有Lewis(x)(Le(x))-三糖的乳酸-n-fucopentaose III （LNFPIII）的增殖反应和白细胞介素(IL)-10反应在感染曼氏血吸虫的人PBMC中进行了评估。所有粪便中卵数低、中、高的患者组均对多价LNFPIII-HSA （HSA =人血清白蛋白）偶联物有反应。所有受试者的PBMC对该糖缀合物均表现出显著的增殖反应。然而，LNFPIII-HSA诱导的白细胞介素(IL)-10水平在鸡蛋数量低和中等组高于鸡蛋数量高的组。可溶性蛋抗原（SEA）也能诱导感染患者PBMC产生IL-10。有趣的是，用游离的LNFPIII（单价形式）配体预处理PBMC可显著抑制sea诱导的IL-10的产生。这些经lnfpiii预处理的PBMC在SEA刺激下的增殖水平明显增加。我们提出，观察到的游离LNFPIII引起的旁观者免疫增强是由于IL-10水平的降低，这可能是由于上调了APC上共刺激分子的表达。综合结果表明，在SEA中含有Le(x)的LNFPIII是一个有效的免疫反应表位，通过IL-10对PBMC对这种寄生虫抗原的反应产生负面影响，(C) American Society for Histocompatibility and Immunogenetics, 2000。Elsevier Science Inc.出版。
The proliferative and interleukin (IL)-10 responses to Lacto-n-fucopentaose III (LNFPIII) that contains Lewis(x)(Le(x))-trisaccharide was assessed in PBMC from humans infected with Schistosoma mansoni. All patient groups with low, medium, and high egg counts in their feces responded to polyvalent LNFPIII-HSA (where HSA = human serum albumin) conjugate. PBMC of all subjects showed a significant proliferative response to this sugar conjugate. However, the levels of interleukin (IL)-10 induced by LNFPIII-HSA were higher in groups with low and medium egg counts than those with high egg. Soluble egg antigens (SEA) also induced IL-10 production by PBMC from infected patients. Interestingly, the SEA-induced IL-10 production was remarkably inhibited by pretreatment of PBMC with free ligands of LNFPIII (monovalent form). These LNFPIII-pretreated PBMC displayed appreciable increase in the level of proliferation to SEA stimulation. We propose that the observed bystander immune potentiation rendered by free LNFPIII is due to the reduced IL-10 level which, presumably, up-regulate expression of co-stimulatory molecules on APC, The ensemble of results indicates that the Le(x)-containing LNFPIII is a potent immunoreactive epitope in SEA that negatively influences PBMC response against this parasite antigens via IL-10, (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.