Effects of bisphenol A administration to pregnant mice on serum Ca and intestinal Ca absorption
Effects of bisphenol A administration to pregnant mice on serum Ca and intestinal Ca absorption
复制标题
双酚A对妊娠小鼠血清钙和肠道钙吸收的影响
DOI：
10.1111/j.1740-0929.2011.00947.x
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发表时间：
2012
影响因子：
2
通讯作者：
Kume S.
中科院分区：
文献类型：
--
作者：
Otsuka H;Sugimoto M;Ikeda S;Kume S.
双酚A（BPA）是一种常用于食品储存塑料的异雌激素。本研究旨在探讨双酚A对孕鼠血清钙及肠道和肾脏钙代谢的影响。从交配后6.5至16.5天（dpc），以2 mg或20 mg/kg体重/天的BPA剂量对妊娠小鼠进行给药。 20 mg BPA处理组小鼠血清钙水平在17.5dpc时显著降低，但十二指肠和空肠碱性磷酸酶活性和维生素D受体蛋白表达无显著差异。 在20 mg BPA处理的小鼠中，肾脏中钙结合蛋白（CaBP-9 k）和活性维生素D合成酶（CYP 27 B1）的信使RNA（mRNA）表达增加。 在用20 mg BPA处理的小鼠中，十二指肠和回肠中调节细胞旁转运的occludin和连接粘附分子A（JAM-A）的mRNA表达增加。 而2 mg BPA对血清Ca及Ca代谢相关基因的mRNA表达无影响。 这些结果表明，在妊娠期间以20 mg/kg体重/天的BPA给药会降低分娩前小鼠的血清Ca，这可能部分是由于细胞旁Ca吸收减少。
Bisphenol A (BPA) is a xenoestrogen commonly used in food storage plastics. The present study was conducted to clarify the effects of BPA administration to pregnant mice on serum calcium (Ca) and Ca metabolism of the gut and kidney. From 6.5 to 16.5 days post coitus (dpc), pregnant mice were administered at 2 mg or 20 mg/kg body weight/day of BPA. Serum Ca was decreased in mice treated with 20 mg BPA at 17.5 dpc, but no remarkable differences were detected in the alkaline phosphatase activity and vitamin D receptor protein expression in the duodenum and jejunum. The messenger RNA (mRNA) expressions of calcium binding protein (CaBP‐9k) and active vitamin D synthesis enzyme (CYP27B1) in the kidney were increased in mice treated with 20 mg BPA. The mRNA expressions of occludin and junction adherence molecular A (JAM‐A) in the duodenum and ileum, which regulate paracellular transport, were increased in mice treated with 20 mg BPA. However, the administration of 2 mg BPA had no effect on serum Ca and mRNA expressions of relative genes in Ca metabolism. These results imply that BPA administration at 20 mg/kg body weight/day during pregnancy decreases serum Ca in pre‐delivery mice, which may be partly due to decreased paracellular Ca absorption.