Antioxidants and cognitive training interact to affect oxidative stress and memory in APP/PSEN1 mice.
Antioxidants and cognitive training interact to affect oxidative stress and memory in APP/PSEN1 mice.
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DOI：
10.1179/147683009x423364
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发表时间：
2009-10
影响因子：
3.6
通讯作者：
McDonald MP
中科院分区：
文献类型：
--
作者：
Harrison FE;Allard J;Bixler R;Usoh C;Li L;May JM;McDonald MP
本研究探讨了APP/PSEN 1双转基因阿尔茨海默病小鼠模型中氧化应激、β-淀粉样蛋白和认知能力之间的关系。在两个实验中，向仅含有维生素C的老年APP/PSEN 1小鼠给予长期膳食补充剂（1g/kg饮食，实验1）。1)或与高（750 IU/kg饮食，Expts. 1和2）或更低（400 IU/kg饲料，实验2)维生素E的剂量通过F4-神经前列腺素或丙二醛测量的氧化应激在对照喂养的APP/PSEN 1小鼠的皮质中升高，并通过补充维生素降低至野生型水平。高剂量维生素E与C在降低氧化应激方面不如单独使用维生素C或低EC饮食组合有效。高剂量组合也损害了两种基因型小鼠的水迷宫表现。在实验2中，较低的维生素EC治疗减弱了APP/PSEN 1小鼠的空间记忆缺陷，并改善了野生型小鼠在水迷宫中的表现。在任何一个实验中，抗氧化剂补充都没有减少淀粉样蛋白沉积。
The present study investigated the relationships among oxidative stress, β-amyloid and cognitive abilities in the APP/PSEN1 double-transgenic mouse model of Alzheimer’s disease. In two experiments, long-term dietary supplements were given to aged APP/PSEN1 mice containing vitamin C alone (1g/kg diet, Expt. 1) or in combination with a high (750 IU/kg diet, Expts. 1 and 2) or lower (400 IU/kg diet, Expt. 2) dose of vitamin E. Oxidative stress, measured by F4-neuroprostanes or malondialdehyde, was elevated in cortex of control-fed APP/PSEN1 mice and reduced to wild-type levels by vitamin supplementation. High dose vitamin E with C was less effective at reducing oxidative stress than vitamin C alone or the low EC diet combination. The high-dose combination also impaired water maze performance in mice of both genotypes. In Experiment 2 the lower vitamin EC treatment attenuated spatial memory deficits in APP/PSEN1 mice and improved performance in wild-type mice in the water maze. Amyloid deposition was not reduced by antioxidant supplementation in either experiment.