Activator-dependent transcription from chromatin in vitro involving targeted histone acetylation by p300
Activator-dependent transcription from chromatin in vitro involving targeted histone acetylation by p300
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DOI：
10.1016/s1097-2765(00)00054-x
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发表时间：
2000-09-01
期刊：
影响因子：
16
通讯作者：
Roeder, RG
中科院分区：
文献类型：
--
作者：
Kundu, TK;Palhan, VB;Roeder, RG
转录辅激活因子p300与多种激活因子表现出物理和功能上的相互作用，并含有内在的乙酰转移酶活性，其确切的辅激活因子在核小体组蛋白乙酰化中的作用相对于其他因素的记载很少。在这里，我们发现p300介导了GAL4-VP16从核小体阵列模板上的乙酰辅酶a依赖性转录，这涉及到GAL4-VP16靶向p300和转录前启动子-近端组蛋白乙酰化，并且不同激活因子对p300的亲和力大致与相应水平的p300依赖性转录相关。这些结果表明，激活因子通过直接相互作用将p300招募到核小体模板中，结合的p300至少在一定程度上通过局部组蛋白乙酰化刺激转录。
The transcriptional coactivator p300 shows physical and functional interactions with a diverse group of activators and contains an intrinsic acetyltransferase activity whose exact coactivator functions in the acetylation of nucleosomal histones versus other factors are poorly documented. Here, we show that p300 mediates acetyl-CoA-dependent transcription by GAL4-VP16 from a nucleosomal array template, that this involves p300 targeting by GAL4-VP16 and promoter-proximal histone acetylation prior to transcription, and that the affinities of different activators for p300 roughly correlate with corresponding levels of p300-dependent transcription. These results indicate that activators recruit p300 to nucleosomal templates by direct interactions and that bound p300 stimulates transcription, at least in part, by localized histone acetylation.