ON ROLE OF MICROTUBULES IN MOVEMENT AND ALIGNMENT OF NUCLEI IN VIRUS-INDUCED SYNCYTIA
ON ROLE OF MICROTUBULES IN MOVEMENT AND ALIGNMENT OF NUCLEI IN VIRUS-INDUCED SYNCYTIA
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DOI：
10.1083/jcb.39.3.526
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发表时间：
1968-01-01
影响因子：
7.8
通讯作者：
CHOPPIN, PW
中科院分区：
文献类型：
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作者：
HOLMES, KV;CHOPPIN, PW
副流感病毒 SV5 感染幼仓鼠肾 (BHK21-F) 细胞会导致快速、广泛的细胞融合。延时摄影显示，当细胞融合时，它们的细胞核以 1-2/z/min 的速度直接迁移到合胞体的中心。在源自成纤维细胞 BHK21-F 细胞的合胞体中，细胞核通常排列成长的、紧密堆积的平行行。偏光显微镜显示在这些行核之间并平行于这些行核的双折射材料，电子显微镜显示直径约 250 A 的细胞质微管束和直径约 80 A 的细丝，平行于核行并在这些行核之间。秋水仙碱处理导致 BHK2 IF 细胞微管消失，80-A 丝数量明显增加。尽管经秋水仙碱处理的 SV5 感染细胞融合，但它们的细胞核不会迁移或形成行，而是保持随机分散在合胞质中。 4°C 孵育不会破坏 BHK21-F 细胞中的微管。已经从 SV5 诱导的合胞体中分离出成排的细胞核，并且发现其中的细胞核与微管密切相关，但与其他细胞质结构无关。这些结果表明，微管划分了细胞核迁移的细胞质通道，并且它们也可能参与核运动的机制。
Infection of baby hamster kidney (BHK21-F) cells with the parainfluenza virus SV5 causes rapid and extensive cell fusion. Time-lapse cinematography shows that when cells fuse, their nuclei migrate straight to the center of the syncytium at rates of 1-2/z/min. Nuclei are often arranged in long, tightly packed, parallel rows in syncytia derived from the fibroblastic BHK21-F cells. Polarization microscopy shows birefringent material between and parallel to these rows of nuclei, and electron microscopy shows bundles of cytoplasmic microtubules,~ 250 A in diameter, and filaments,~ 80 A in diameter, parallel to and between the rows of nuclei. Colchicine treatment causes disappearance of microtubules from BHK2 IF cells and an apparent increase in the number of 80-A filaments. Although colchicine-treated, SV5-infected cells fuse, their nuclei do not migrate or form rows but remain randomly scattered through the syncytial cytoplasm. Incubation at 4 C does not disrupt microtubules in BHK21-F cells. Rows of nuclei have been isolated from SV5-induced syncytia, and the nuclei in them have been found to be intimately associated with microtubules but not with other cytoplasmic structures. These results suggest that microtubules demarcate cytoplasmic channels through which nuclei migrate and that they may also be involved in the mechanism of nuclear movement.