MoMon1 is required for vacuolar assembly, conidiogenesis and pathogenicity in the rice blast fungus Magnaporthe oryzae
MoMon1 is required for vacuolar assembly, conidiogenesis and pathogenicity in the rice blast fungus Magnaporthe oryzae
复制标题
MoMon1 是稻瘟病菌 Magnaporthe oryzae 液泡组装、分生孢子发生和致病性所必需的
DOI：
10.1016/j.resmic.2013.01.001
复制
发表时间：
2013-05-01
影响因子：
2.6
通讯作者：
Liu, Xiao-Hong
中科院分区：
文献类型：
--
作者：
Gao, Hui-Min;Liu, Xiao-Guang;Liu, Xiao-Hong
Mon1蛋白参与胞质到液泡的运输、液泡形态和自噬，并且是酿酒酵母中同型液泡融合所必需的。在这里，我们确定MoMON1从Magnaporthe muscle作为S的直系同源物。酿酒酵母MON1，对液泡和囊泡融合的形态至关重要。MoMON1的靶基因缺失导致菌丝中小点状空泡的积累和对莫能菌素（一种阻断细胞内蛋白质转运的抗生素）的超敏反应。Delta Momon1突变体表现出显着减少气生菌丝发育和不良分生孢子。DeltaMomon1分生孢子能分化附着胞。然而，Delta Momon1是非致病性的水稻叶片，即使在伤口接种。此外，Delta Momon 1对刚果红和SDS有轻微的超敏感性，但对细胞壁降解酶不敏感，这表明其细胞壁发生了显著变化。自噬过程在Delta Momon1突变体中被阻断。综上所述，我们的研究结果表明MoMON1在M.米。(c)2013年巴斯德研究所。由Elsevier Masson SAS出版。All rights reserved.
Mon1 protein is involved in cytoplasm-to-vacuole trafficking, vacuolar morphology and autophagy, and is required for homotypic vacuole fusion in Saccharomyces cerevisiae. Here we identify MoMON1 from Magnaporthe oryzae as an ortholog of S. cerevisiae MON1, essential for the morphology of the vacuole and vesicle fusion. Target gene deletion of MoMON1 resulted in accumulation of small punctuate vacuoles in the hypha and hypersensitivity to monensin, an antibiotic that blocks intracellular protein transport. The Delta Momon1 mutant exhibited significantly reduced aerial hyphal development and poor conidiation. Conidia of Delta Momon1 were able to differentiate appressoria. However, Delta Momon1 was non-pathogenic on rice leaves, even after wound inoculation. In addition, Delta Momon1 was slightly hypersensitive to Congo red and SDS, but not to cell wall degrading enzymes, suggesting significant alterations in its cell wall. The autophagy process was blocked in the Delta Momon1 mutant. Taken together, our results suggest that MoMON1 has an essential function in vacuolar assembly, autophagy, fungal development and pathogenicity in M. oryzae. (c) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.