BD™ PuraMatrix™ peptide hydrogel seeded with Schwann cells for peripheral nerve regeneration
BD™ PuraMatrix™ peptide hydrogel seeded with Schwann cells for peripheral nerve regeneration
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DOI：
10.1016/j.brainresbull.2010.07.001
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发表时间：
2010-10-30
影响因子：
3.8
通讯作者：
Wiberg, Mikael
中科院分区：
文献类型：
--
作者：
McGrath, Aleksandra M.;Novikova, Liudmila N.;Wiberg, Mikael
本研究观察了人工合成基质BD(TM)PuraMatrix(TM)多肽(BD)和培养的雪旺细胞(Schwann细胞)填充的膜导管对周围神经损伤后再生的影响。坐骨神经切断后，用BD水凝胶或含有雪旺细胞的BD水凝胶超滤膜管道桥接神经残端之间10 mm的间隙。在对照实验中，神经缺损的修复采用海藻酸盐/纤维连接蛋白水凝胶或自体神经移植管道内的神经管道，3周后评估脊髓运动神经元的再生和肌肉重量的恢复。与海藻酸盐/纤维连接蛋白水凝胶和海藻酸盐/纤维连接蛋白复合雪旺细胞组相比，海藻酸盐/纤维连接蛋白水凝胶和海藻酸盐/纤维连接蛋白复合雪旺细胞水凝胶组再生轴突的距离明显增加，轴突穿过损伤间隙进入远端神经残端，BD水凝胶导管内神经纤维和雪旺细胞呈线性排列。与神经移植相比，海藻酸盐水凝胶和海藻酸盐/纤维连接蛋白水凝胶组腓肠肌再生运动神经元的数量和重量的恢复都较差。提示：BD水凝胶和海藻酸盐/纤维连接蛋白水凝胶填充神经移植后，再生运动神经元的数量和重量的恢复均低于神经移植组含有雪旺细胞的BD水凝胶可用于生物合成管道中，以提高神经缺损的轴突再生率(C)2010爱思唯尔公司版权所有。
This study investigated the effects of a membrane conduit filled with a synthetic matrix BD (TM) PuraMatrix (TM) peptide (BD) hydrogel and cultured Schwann cells on regeneration after peripheral nerve injury in adult ratsAfter sciatic axotomy a 10 mm gap between the nerve stumps was bridged using ultrafiltration membrane conduits filled with BD hydrogel or BD hydrogel containing Schwann cells In control experiments the nerve defect was bridged using either membrane conduits with alginate/fibronectin hydrogel or autologous nerve graft Axonal regeneration within the conduit was assessed at 3 weeks and regeneration of spinal motoneurons and recovery of muscle weight evaluated at 16 weeks postoperativelySchwann cells survived in the BD hydrogel both in culture and after transplantation into the nerve defect Regenerating axons grew significantly longer distances within the conduits filled with BD hydrogel when compared with the alginate/fibronectin hydrogel and alginate/fibronectin with Schwann cells Addition of Schwann cells to the BD hydrogel considerably Increased regeneration distance with axons crossing the injury gap and entering into the distal nerve stump The conduits with BD hydrogel showed a linear alignment of nerve fibers and Schwann cellsThe number of regenerating motoneurons and recovery of the weight of the gastrocnemius muscle was inferior in BD hydrogel and alginate/fibronectin groups compared with nerve grafting Addition of Schwann cells did not improve regeneration of motoneurons or muscle recoveryThe present results suggest that BD hydrogel with Schwann cells could be used within biosynthetic conduits to Increase the rate of axonal regeneration across a nerve defect (C) 2010 Elsevier Inc All rights reserved.