Extremely high molecular diversity within the East Asian nematode Caenorhabditis sp. 5
Extremely high molecular diversity within the East Asian nematode Caenorhabditis sp. 5
复制标题
东亚线虫 Caenorhabditis sp. 内具有极高的分子多样性。
DOI：
10.1111/j.1365-294x.2010.04862.x
复制
发表时间：
2010-11-01
影响因子：
4.9
通讯作者：
Cutter, Asher D.
中科院分区：
文献类型：
--
作者：
Wang, Guo-Xiu;Ren, Shuang;Cutter, Asher D.
大多数自体雌雄同体线虫模式生物秀丽隐杆线虫的亲属通过雄性和雌性之间的专性远系繁殖进行繁殖，这也代表了该属内的祖先繁殖模式。然而，很少有人知道的遗传多样性和分化的范围内，这些gonochoristic物种，特别是那些温带欧洲和北美以外的发现。这是至关重要的，以了解这些物种的进化过程中运作，以提供一个框架，破译雌雄同体的进化和应用的基线的异型小杆线虫的进化遗传学的问题。在这里，我们调查的第一次的分子序列变异的小杆线虫。5，一种常见于东亚。我们确定了巨大的水平的常设遗传变异的海洋广播产卵海鞘，玻璃海鞘中观察到的水平。虽然我们的文件显着隔离的距离，我们证明，高多态性内C。sp.5的分离不是由于居群间的分化或隐种的存在。这些研究结果表明，分子群体遗传学的方法来研究专性远交种的小杆线虫将被证明是强大的识别和表征功能和进化的重要功能的基因组。
Most relatives of the self-fertilizing hermaphroditic nematode model organism Caenorhabditis elegans reproduce via obligate outbreeding between males and females, which also represents the ancestral mode of reproduction within the genus. However, little is known about the scope of genetic diversity and differentiation within such gonochoristic species, especially those found outside of temperate Europe and North America. It is critical to understand the evolutionary processes operating in these species to provide a framework for deciphering the evolution of hermaphroditism and a baseline for the application of outcrossing Caenorhabditis to problems in evolutionary genetics. Here, we investigate for the first time molecular sequence variation for Caenorhabditis sp. 5, a species found commonly in eastern Asia. We identify enormous levels of standing genetic variation that approach the levels observed in the marine broadcast-spawning sea squirt, Ciona savignyi. Although we document significant isolation by distance, we demonstrate that the high polymorphism within C. sp. 5 is not because of strong differentiation among populations or to the presence of cryptic species. These findings illustrate that molecular population genetic approaches to studying obligately outbreeding species of Caenorhabditis will prove powerful in identifying and characterizing functionally and evolutionarily important features of the genome.