Biodegradation of anthracene and phenanthrene by bacteria isolated from oil-contaminated soil of Bangladesh
Biodegradation of anthracene and phenanthrene by bacteria isolated from oil-contaminated soil of Bangladesh
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DOI：
10.1080/02757540.2017.1382481
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发表时间：
2017-01-01
影响因子：
2.5
通讯作者：
Uddin, Mohammad
中科院分区：
文献类型：
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作者：
Chowdhury, Rocky;Dhar, Kartik;Uddin, Mohammad
多环芳烃（Polycyclicaromatic hydrocarbons，PAHs）是一种普遍存在的环境污染物，通过自然和人为活动进入环境。由于它们对包括人类在内的各种生命形式具有毒性，因此多环芳烃的解毒对于减少其对细胞的影响至关重要。在这项研究中，我们已经分离出两种细菌能够降解两种常见的多环芳烃，蒽和菲，从污染的土壤样品，通过使用选择性富集培养补充测试多环芳烃作为唯一的碳源和能源。根据16 SrRNA基因序列分析，将分离的细菌分别鉴定为铜绿假单胞菌KD株和嗜麦芽窄食单胞菌RC株。蒽和菲的降解是通过测量氧化还原指示剂2，6-二氯酚靛酚的脱色程度来间接估计的，掺入到添加PAH的矿物盐介质中。在蒽的情况下，铜绿假单胞菌和S.嗜麦芽窄食症。另一方面，铜绿假单胞菌和S. maltophilia，分别在菲的利用。
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants entering into the environment through natural and anthropogenic activities. Owing to their toxicity to various life forms including humans, detoxification of PAHs is crucial to reduce their effects on cells. In this study, we have isolated two bacteria capable of degrading two common PAHs, anthracene and phenanthrene, from contaminated soil samples by using selective enrichment culture supplemented with test PAHs as the sole source of carbon and energy. The isolated bacteria were identified and affiliated as Pseudomonas aeruginosa strain KD and Stenotrophomonas maltophilia strain RC based on their 16s rRNA gene sequences. The degradation of anthracene and phenanthrene was estimated indirectly by measuring the decolourisation extent of a redox indicator, 2, 6-dichlorophenolindophenol, incorporated into PAH-supplemented mineral salt media. In the case of anthracene, >= 90% decolourisation was recorded at 20 and 48 days for P. aeruginosa and S. Maltophilia, respectively. On the other hand, >= 94% decolourisation was recorded at 56 and 52 days for P. aeruginosa and S. maltophilia, respectively during the utilisation of phenanthrene.