Mitochondrial behaviour in the early stages of ROS stress leading to cell death in Arabidopsis thaliana.
Mitochondrial behaviour in the early stages of ROS stress leading to cell death in Arabidopsis thaliana.
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DOI：
10.1093/aob/mci181
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发表时间：
2005-08
期刊：
影响因子：
4.2
通讯作者：
Keiko Yoshinaga;S. Arimura;Y. Niwa;N. Tsutsumi;H. Uchimiya;M. Kawai‐Yamada
中科院分区：
文献类型：
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作者：
Keiko Yoshinaga;S. Arimura;Y. Niwa;N. Tsutsumi;H. Uchimiya;M. Kawai‐Yamada
背景和目的活性氧（ROS）参与触发细胞死亡.为了可视化ROS胁迫下的线粒体行为，研究了具有细胞靶向GFP（S65T）的转基因拟南芥植物。方法用活性氧和活性氧诱导剂过氧化氢、百草枯和甲萘醌处理拟南芥叶片。使用共聚焦激光扫描显微镜系统进行显微镜观察，并监测电解质渗漏。关键结果处理后，线粒体显示出从杆状到圆形的形态学变化。与对照组相比，用H2O2处理的线粒体的大小减少了一半。同时，细胞质流动受阻，线粒体最终肿胀。丁二酮单肟，肌球蛋白ATP酶的抑制剂，处理叶片，导致类似的行为的线粒体下ROS胁迫。结论线粒体形态学改变与细胞质流动的停止可能存在相互作用，这是ROS应激导致细胞死亡的特征之一。
BACKGROUND AND AIMS Reactive oxygen species (ROS) are involved in triggering cell death. To visualize mitochondrial behaviour under ROS stress, transgenic arabidopsis plants possessing mitochondrial-targeted GFP (S65T) were studied. METHODS Arabidopsis leaves were treated with ROS and ROS-inducing chemicals such as hydrogen peroxide, paraquat and menadione. Microscopic observations were carried out using a confocal laser scanning microscope system, and electrolyte leakage was also monitored. KEY RESULTS After treatment, mitochondria showed morphological changes from a bacillus-like to a round shape. The size of mitochondria treated with H(2)O(2) decreased by half compared with controls. Concurrently, cytoplasmic streaming was blocked and mitochondria eventually swelled. Treatment of leaves with butanedione monoxime, an inhibitor of myosin ATPase, resulted in similar behaviour of mitochondria to that under ROS stress. CONCLUSIONS The results indicate that morphological changes of mitochondria and cessation of cytoplasmic streaming may interact, and this phenomenon is one of the features of ROS stress-induced cell death.