Time-course of the uterine response to estradiol-17beta in ovariectomized ewes: expression of angiogenic factors.
Time-course of the uterine response to estradiol-17beta in ovariectomized ewes: expression of angiogenic factors.
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DOI：
10.1095/biolreprod59.3.613
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发表时间：
1998-09
影响因子：
3.6
通讯作者：
L. Reynolds;J. Kirsch;K. C. Kraft;D. Redmer
中科院分区：
文献类型：
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作者：
L. Reynolds;J. Kirsch;K. C. Kraft;D. Redmer
子宫血管生成因子（血管内皮生长因子[VEGF]和碱性成纤维细胞生长因子[bFGF]）的表达进行了评价，在卵巢切除母羊在0，2，4，8，24，48，或72小时后雌二醇（E2）治疗。子宫内膜VEGF mRNA从0到4小时增加超过5倍，在8小时保持升高，然后下降到72小时后E2治疗。相反，子宫内膜bFGF mRNA在0 ~ 4 h内保持不变，4 ~ 8 h增加2.2倍，24 h仍保持升高，然后下降至72 h。VEGF的免疫染色存在于子宫肌层和子宫内膜微血管（小动脉，小静脉，和/或毛细血管），也在子宫肌层平滑肌; VEGF的免疫染色的模式，随后的mRNA表达，被升高，在4和8小时后E2治疗。bFGF的免疫染色只存在于子宫腺; bFGF的免疫染色模式也遵循其mRNA，在E2后8和24小时升高。根据这些观察结果，我们认为，VEGF和bFGF可能是重要的因素，负责戏剧性的子宫微血管反应发生后8至24小时E2治疗卵巢切除母羊。
Uterine expression of angiogenic factors (vascular endothelial growth factor [VEGF] and basic fibroblast growth factor [bFGF]) was evaluated in ovariectomized ewes at 0, 2, 4, 8, 24, 48, or 72 h after estradiol (E2) treatment. Endometrial VEGF mRNA increased more than 5-fold from 0 to 4 h, remained elevated at 8 h, and then declined through 72 h after E2 treatment. In contrast, endometrial bFGF mRNA remained constant from 0 to 4 h, increased 2.2-fold from 4 to 8 h, remained elevated at 24 h, and then declined through 72 h. Immunostaining for VEGF was present in myometrial and endometrial microvessels (arterioles, venules, and/or capillaries) and also in myometrial smooth muscle; the pattern of VEGF immunostaining followed that of mRNA expression, being elevated at 4 and 8 h after E2 treatment. Immunostaining for bFGF was present exclusively in uterine glands; the pattern of bFGF immunostaining also followed that of its mRNA, being elevated at 8 and 24 h after E2. On the basis of these observations, we suggest that VEGF and bFGF are probably important factors responsible for the dramatic uterine microvascular response that occurs 8 to 24 h after E2 treatment in ovariectomized ewes.