ISOLATION AND CHARACTERIZATION OF MICROCIN E-492 FROM KLEBSIELLA-PNEUMONIAE
ISOLATION AND CHARACTERIZATION OF MICROCIN E-492 FROM KLEBSIELLA-PNEUMONIAE
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DOI：
10.1007/bf00692715
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发表时间：
1984-01-01
影响因子：
2.8
通讯作者：
DELORENZO, V
中科院分区：
文献类型：
--
作者：
DELORENZO, V
粪源肺炎克雷伯氏菌RYC492产生了一种可透析性的多肽类抗菌素微球菌素E492。本文介绍了一种从产生菌液体培养物中提取该抗生素的方法。该方法基于微球素在疏水基质Bondapak C18上的定量保留，得到了适合于进一步高压液相层析纯化的高活性色素和无盐浓缩物。纯化后的微球蛋白E492是一种碱性疏水性多肽，表观分子质量约为5000，耐酸耐热，在微量介质中比在富介质中活性高。关于微生物在肠道微生物生态系统中可能的生态作用，本文讨论了这些特性。
The production of a dialysable peptidic antibacterial named microcin E492 by the strain of faecal originKlebsiella pneumoniaeRYC492 has previously been reported. In this paper, a procedure to extract this antibiotic from liquid cultures of the producer strain is described. This method was based in the quantitative retention of the microcin on the hydrophobic matrix Bondapak C18 and led to highly active pigment- and salf-free concentrates appropriate for further purification by high pressure liquid chromatography. The characterization of purified preparations indicated that microcin E492 was a basic and hydrophobic peptide with an apparent molecular mass of about 5,000, acid- and heat-resistant and much more active in minimal than in rich medium. These properties are discussed with regard to the likely ecological role of the microcin in the microbial ecosystem of the intestine.