TOPOGRAPHICAL DISTRIBUTION OF SULFATED GLYCOSAMINOGLYCANS IN HUMAN TEMPOROMANDIBULAR-JOINT DISKS - HISTOCHEMICAL STUDY OF AN AUTOPSY MATERIAL
TOPOGRAPHICAL DISTRIBUTION OF SULFATED GLYCOSAMINOGLYCANS IN HUMAN TEMPOROMANDIBULAR-JOINT DISKS - HISTOCHEMICAL STUDY OF AN AUTOPSY MATERIAL
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DOI：
10.1111/j.1600-0714.1976.tb01775.x
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发表时间：
1976-01-01
影响因子：
3.3
通讯作者：
KOPP, S
中科院分区：
文献类型：
--
作者：
KOPP, S
应用组织化学方法研究了18例人颞下颌关节盘内硫酸化糖胺聚糖（GAG）的分布及其与性别、年龄和骨关节病的关系。将盘包埋在石蜡中，并在不同水平切割5-7 μ m厚的额切片。使用两种染色方法，甲苯胺蓝在pH 0.5和阿辛蓝与不同浓度的MgCl 2。两种方法的结果相当。硫酸化GAG（以pH 0.5的甲苯胺蓝异染染色和MgCl 2浓度高于0.55 M的阿辛蓝染色为代表）出现在椎间盘的中央承重部分，均匀分布在内侧-外侧方向。阿辛蓝染色法的结果表明存在硫酸化GAG，其特征与软骨素/硫酸皮肤素和硫酸角质素相似。后一种发现在主要位于盘中心部分的表面区域（10-100 μ m）中最常见。在被判定为早期骨关节病的椎间盘的肉眼可见薄区域中，观察到硫酸化GAG染色显著减少。在正常或骨关节病椎间盘中，未发现GAG分布的年龄或性别差异。
The distribution of sulfated glycosaminoglycans (GAG) in the human temporomandibular joint disk and its relationship to sex, age and osteoarthrosis was studied by histochemical methods in autopsy material from 18 individuals. The disks were embedded in paraffin and frontal sections, 5-7 .mu.m thick, were cut at different levels. Two staining methods were used, toluidine blue at pH 0.5 and alcian blue with different concentrations of MgCl2. The 2 methods gave comparable results. The sulfated GAG as represented by metachromatic staining with toluidine blue at pH 0.5 and staining with alcian blue in concentrations of MgCl2 above 0.55 M were found in the central load-bearing part of the disks evenly distributed in the medio-lateral direction. The findings from the alcian blue staining method indicated the presence of sulfated GAG with characteristics similar to chondroitin/dermatan sulfate and keratan sulfate. The latter finding was most frequent in a surface zone (10-100 .mu.m) located mainly in the central part of the disks. In macroscopically thin areas of the disks judged as early osteoarthrosis, a significant reduction in the staining of sulfated GAG was observed. No age or sex differences were found in the distribution of GAG, either in normal or in osteoarthrotic disks.