Osteopontin: a component of matrix in capsular opacification and subcapsular cataract.
Osteopontin: a component of matrix in capsular opacification and subcapsular cataract.
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DOI：
10.1167/iovs.02-0873
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发表时间：
2003-04
影响因子：
4.4
通讯作者：
S. Saika;T. Miyamoto;I. Ishida;Y. Ohnishi;A. Ooshima
中科院分区：
文献类型：
--
作者：
S. Saika;T. Miyamoto;I. Ishida;Y. Ohnishi;A. Ooshima
目的检测人晶状体囊膜混浊和囊下白内障组织中是否含有骨桥蛋白，以及小鼠透镜上皮细胞损伤后是否表达骨桥蛋白。方法采用免疫组织化学方法检测人晶状体囊膜及前囊下白内障手术后囊膜组织中骨桥蛋白的表达。然后在囊切开后愈合的小鼠透镜的上皮中确定骨桥蛋白的空间和时间蛋白表达模式。结果白内障手术后10天玻璃体切除术时提取的标本中的人透镜上皮细胞以及较长愈合间隔后的标本中的人晶状体上皮细胞被抗骨桥蛋白抗体标记，而未损伤的透镜上皮细胞则未被标记。在愈合后期，囊膜混浊的基质为骨桥蛋白阳性。前囊下白内障组织中的透镜细胞也呈阳性。骨桥蛋白在透镜细胞表面、胞膜、胞浆及基质中均有表达。与正常的未损伤标本不同，一些愈合的术后标本的前透镜囊和前囊下白内障标本的骨桥蛋白也呈微弱或弱染色。小鼠透镜上皮细胞在损伤后8 h开始表达骨桥蛋白，此时细胞形态尚未由上皮细胞型转变为成纤维细胞型。骨桥蛋白的表达持续在愈合期间，甚至在细胞转化为成纤维细胞样细胞。结论人晶状体后囊膜混浊和前囊下白内障的细胞外基质中含有骨桥蛋白。小鼠透镜的上皮细胞也异位表达骨桥蛋白以响应囊损伤。
PURPOSE To examine whether tissues of human capsular opacification and subcapsular cataract contain osteopontin, an adhesive matrix protein, and whether mouse lens epithelium expresses osteopontin after injury. METHODS An immunohistochemical examination was conducted to determine whether matrices in human postoperative capsular specimens and anterior subcapsular cataract contain osteopontin. The spatial and temporal protein expression patterns of osteopontin were then determined in epithelium of a healing mouse lens after a capsular incision. RESULTS Human lens epithelial cells in the specimens extracted at the time of vitrectomy 10 days after cataract surgery and also after longer healing intervals were labeled with an anti-osteopontin antibody, whereas uninjured lens epithelium was not. In the later healing phase, matrix of capsular opacification was positive for osteopontin. Lens cells amid anterior subcapsular cataract tissue were also positive. Osteopontin was detected in the cell surface and membrane and the cytoplasm of lens cells, as well as in the matrix. Unlike normal uninjured specimens, anterior lens capsule of some of the healing postoperative specimens and anterior subcapsular cataract specimens also faintly or weakly stained for osteopontin. Mouse lens epithelium started to express osteopontin protein at 8 hours after injury, before the cells changed their shape from epithelial cell type to fibroblast type. Expression of osteopontin lasted during the healing interval, even after the cells transformed into fibroblast-like cells. CONCLUSIONS Extracellular matrix in human postoperative capsular opacification and anterior subcapsular cataract contains osteopontin. Epithelial cells of a mouse lens also ectopically express osteopontin in response to capsular injury.