Multipotency of melanoblasts isolated from murine skin depends on the Notch signal pathway.
Multipotency of melanoblasts isolated from murine skin depends on the Notch signal pathway.
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从小鼠皮肤中分离出的成黑细胞的多能性取决于 Notch 信号通路。
DOI：
10.1002/dvdy.24385
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
and Takahiro Kunisada
中科院分区：
文献类型：
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作者：
Natsuki Watanabe;Tsutomu Motohashi;Masahiro Nishioka;Norito Kawamura;Tomohisa Hirobe;and Takahiro Kunisada
背景：黑素母细胞(MBS)来源于神经脊细胞，在体内只能分化为黑素细胞(Ms)。利用Sox10-IRES-Venus小鼠和小鼠胚胎干细胞，我们研究了MBS是如何表达其多能性的。结果：在ST2基质细胞存在的情况下，MBS产生了含有NS、Gs和MS的集落，但当与角质形成细胞或ST2培养上清液孵育时，它们只产生M集落，这表明MBS需要与ST2基质细胞接触才能表达其多能性。Notch信号被认为是MBS通过细胞间接触维持和分化的重要信号之一。当Notch信号被抑制时，MBS主要产生仅含有NS、Gs或ms一种细胞的集落；即使在ST2基质细胞上，含有两种或三种细胞的集落数量也明显减少，显示其分化潜能受到限制。而当Notch信号被激活时，含有两种或三种细胞的集落数量增加，表明它们的多能性得到了维持。结论：Notch信号在MB多能性中发挥了新的作用。发育动力学，2016。©2015 Wiley期刊，Inc.
Background: Melanoblasts (MBs), derived from neural crest cells, only differentiate into melanocytes (Ms) in vivo. We previously showed that MBs isolated from mouse skin were multipotent, generating neurons (Ns) and glial cells (Gs) together with Ms. Using Sox10‐IRES‐Venus mice and mouse embryonic stem cells, we investigated how MBs expressed their multipotency.Results: MBs generated colonies containing Ns, Gs, and Ms in the presence of ST2 stromal cells, but they generated only M colonies when incubated with keratinocytes or ST2 culture supernatant, thus showing that MBs required contact with ST2 stromal cells for expression of their multipotency. Notch signaling was shown to be one of the important cues for the maintenance and differentiation of MBs through cell–cell contact. When Notch signaling was inhibited, MBs mainly generated colonies that contained just one type of cells, Ns, Gs, or Ms; the number of colonies containing two or three types of cells markedly decreased even on ST2 stromal cells, showing restriction of their differentiation potency. Whereas when Notch signaling was activated, the number of colonies containing two or three types of cells increased, indicating that their multipotency had been maintained.Conclusions: Our results demonstrate that Notch signaling played novel roles in MB multipotency.Developmental Dynamics 245:460–471, 2016. © 2015 Wiley Periodicals, Inc.