Bridging the gap - Biocompatibility of microelectronic materials
Bridging the gap - Biocompatibility of microelectronic materials
复制标题
DOI：
10.1016/j.actbio.2005.10.006
复制
发表时间：
2006-03-01
影响因子：
9.7
通讯作者：
Wanzenboeck, HD
中科院分区：
文献类型：
--
作者：
Bogner, E;Dominizi, K;Wanzenboeck, HD
人们对基于细胞的微电子生物传感器越来越感兴趣，用于对生物技术管道中的新产品进行高通量筛选。这需要对用作细胞生长支撑的材料的生物相容性有基本了解。使用人类来源的单层 Caco-2 细胞，评估了涂有各种金属、电介质和半导体的硅晶片的生物相容性。除了显微镜检查之外，还选择指示活力的细胞增殖以及指示贴壁生长细胞分化的刷状缘酶活性作为估计生物相容性的参数。用于沉积涂层的晶片类型最初会影响最终产品的生物相容性。 p 掺杂硅具有完全生物相容性，而 n 掺杂硅则降低了细胞增殖。在不同的涂层之中。与玻璃相比，铝和钛甚至可以促进细胞生长。在涂层晶圆上培养细胞 6 天表明，Ti 和 ZrO2 涂层晶圆上粘附细胞的分化与玻璃相当，而 Si3N4、Au、Al 和 ITO 涂层将分化降低至 15-35%。对于 Au 和 Si3N4，这种效应随着培养时间的延长而达到平衡。这些结果证明了仔细选择用于生产基于细胞的生物传感器的材料的重要性。 (c) 2005 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
There is an increasing interest in cell-based microelectronic biosensors for high-throughput screening of new products from the biotech pipeline. This requires fundamental knowledge of the biocompatibility of the materials used as the g-rowing support for the cells. Using monolayer-forming Caco-2 cells of human origin, the biocompatibility of silicon wafers coated with various metals, dielectrics and semiconductors was assessed. Besides microscopic inspection, proliferation of cells indicating viability as well as brush border enzyme activity indicating differentiation of adherent growing cells were chosen as parameters to estimate biocompatibility. The type of wafer used for deposition of the coating initially influences the biocompatibility of the final product. Whereas p-doped silicon was fully biocompatible, n-doped silicon reduced the proliferation of cells. Among the different coatings. Al and Ti even increased the cell growth as compared to glass. Culturing the cells for 6 days on coated wafers demonstrated that the differentiation of adhering cells on Ti- and ZrO2-coated wafers was comparable to glass, whereas coatings with Si3N4, Au, Al, and ITO reduced differentiation to 15-35%. In the cases of Au and Si3N4 this effect equilibrated with prolonged culturing. These results demonstrate the importance of a careful selection of the materials used for the production of cell-based biosensors. (c) 2005 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.