A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male-sterile rice
A pentatricopeptide repeat-containing gene that promotes the processing of aberrant atp6 RNA of cytoplasmic male-sterile rice
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DOI：
10.1016/s0014-5793(03)00480-0
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发表时间：
2003-06-05
期刊：
影响因子：
3.5
通讯作者：
Toriyama, K
中科院分区：
文献类型：
--
作者：
Kazama, T;Toriyama, K
水稻胞质雄性不育（CMS）的恢复基因（Rf-1）是线粒体atp 6异常RNA的加工基因。我们已经进行了图位克隆的Rf-1基因，发现一个4.7 kb的恢复系的基因组片段，促进异常atp 6 RNA的加工时，引入到CMS系。该基因组片段含有一个单一的开放阅读框，编码35个氨基酸的五肽重复序列（PPR）的18个重复序列。克隆的PPR基因可能是Rf-1的候选基因。一个非恢复基因型被确定为在编码区内有缺失。(C)2003年由Elsevier Science B. V.代表欧洲生物化学学会联合会出版。
A fertility restorer gene (Rf-1) of [ms-bo] cytoplasmic male sterility (CMS) in rice has been reported to be responsible for the processing of RNA of aberrant atp6 of mitochondria. We have carried out map-based cloning of the Rf-1 gene and found that a 4.7-kb genomic fragment of a restorer line promoted the processing of aberrant atp6 RNA when introduced into a CMS line. The genomic fragment contained a single open reading frame encoding 18 repeats of the 35 amino acid pentatricopeptide repeat (PPR) motif. The cloned PPR gene is a possible candidate of Rf-1. A non-restoring genotype was identified to have deletions within the coding region. (C) 2003 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.