Developmental regulation of the human relaxin genes in the decidua and placenta: overexpression in the preterm premature rupture of the fetal membranes.
Developmental regulation of the human relaxin genes in the decidua and placenta: overexpression in the preterm premature rupture of the fetal membranes.
复制标题
蜕膜和胎盘中人类松弛素基因的发育调节：早产胎膜早破中的过度表达。
DOI：
10.1095/biolreprod57.4.908
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发表时间：
1997
影响因子：
3.6
通讯作者：
Bryant-Greenwood,GD
中科院分区：
文献类型：
--
作者：
Bogic,LV;Yamamoto,SY;Millar,LK;Bryant-Greenwood,GD
蜕膜和胎盘合成人松弛素，称为H1和H2，据信以自分泌/旁分泌方式参与羊膜和绒毛膜中的胶原重塑。松弛素基因的发育调控在正常妊娠中进行定量的6个48-mer的寡核苷酸探针，检测松弛素基因的原位杂交组织化学。在妊娠12.5-14.4周，松弛素在蜕膜（p< 0.01）和胎盘（p< 0.05）中的表达均显著增加，胎盘中的平均峰值是蜕膜的两倍多，提示松弛素基因的协调调节。在自然分娩和分娩后，蜕膜和胎盘松弛素基因表达的边际增加occursed.Given这些正常的数据，三个异常早产的情况下进行了调查：1）未提前破膜的子宫过早收缩，2）胎膜早破（PPROM），3）剖宫产的原因与完整的膜和无宫缩。清除显示宫内感染的组织。PPROM患者的早产蜕膜中松弛素的表达显著高于第1组（p< 0.02）或第3组（p< 0.008）。这些数据通过使用松弛素cRNA探针的北方分析证实。PPROM后胎盘组织中胎盘合体滋养层松弛素的表达也明显增高，且表达水平一致。相比之下，在足月时收集的组织显示蜕膜或胎盘没有这种增加。
The decidua and placenta synthesize the human relaxins, termed H1 and H2, believed to be involved in collagen remodeling in the amnion and chorion in an autocrine/paracrine manner. The developmental regulation of the relaxin genes was quantitated in normal pregnancy by in situ hybridization histochemistry with six 48-mer oligonucleotide probes that detect both relaxin genes. A significant increase in relaxin expression occurred in both decidua (p< 0.01) and placenta (p< 0.05) at 12.5–14.4 wk gestation, with the mean peak value in the placenta more than double that of the decidua, suggesting a coordinate regulation of the relaxin genes. At term after spontaneous labor and delivery, a marginal increase in both decidual and placental relaxin gene expression occurred.Given these normal data, three abnormal preterm situations were investigated: 1) premature uterine contractions without prior rupture of the membranes, 2) premature rupture of the fetal membranes (PPROM), 3) cesarean section for medical reasons with intact membranes and no uterine contractions. Tissues showing intrauterine infection were eliminated. Significantly more relaxin was expressed in the preterm decidua from patients with PPROM when compared to patients in group 1 (p< 0.02) or group 3 (p< 0.008). These data were confirmed by Northern analysis with a relaxin cRNA probe. The placental tissues after PPROM also had a significantly higher and a uniform overexpression of relaxin in the placental syncytiotrophoblast. Tissues collected at term, in comparison, showed no such increases in decidua or placenta.